Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

A. E. Wakefield, S. P. Keely, J. R. Stringer, C. B. V. Christensen, Peter Ahrens, S. E. Peters, Vivi Bille-Hansen, Sven Erik Lind Jorsal, S. A. Henriksen, O.P. Settnes

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.
    Original languageEnglish
    JournalActa Pathologica Microbiologica et Immunologica Scandinavica
    Volume105
    Issue number4
    Pages (from-to)317-321
    ISSN0903-4641
    Publication statusPublished - 1997

    Keywords

    • nucleotide sequence
    • Pneumocystis carinii
    • DNA amplification
    • pig

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