Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

A. E. Wakefield; S. P. Keely; J. R. Stringer; C. B. V. Christensen; Peter Ahrens; S. E. Peters; Vivi Bille-Hansen; Sven Erik Lind Jorsal; S. A. Henriksen; O.P. Settnes

Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

A. E. Wakefield, S. P. Keely, J. R. Stringer, C. B. V. Christensen, Peter Ahrens, S. E. Peters, Vivi Bille-Hansen, Sven Erik Lind Jorsal, S. A. Henriksen, O.P. Settnes

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.

Original language	English
Journal	Acta Pathologica Microbiologica et Immunologica Scandinavica
Volume	105
Issue number	4
Pages (from-to)	317-321
ISSN	0903-4641
Publication status	Published - 1997

Keywords

nucleotide sequence
Pneumocystis carinii
DNA amplification
pig

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Wakefield, A. E. ; Keely, S. P. ; Stringer, J. R. ; Christensen, C. B. V. ; Ahrens, Peter ; Peters, S. E. ; Bille-Hansen, Vivi ; Jorsal, Sven Erik Lind ; Henriksen, S. A. ; Settnes, O.P. / Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification. In: Acta Pathologica Microbiologica et Immunologica Scandinavica. 1997 ; Vol. 105, No. 4. pp. 317-321.

@article{d94f0e84d800463c9afda544640e907e,

title = "Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification",

abstract = "DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.",

keywords = "nucleotide sequence, Pneumocystis carinii, DNA amplification, pig",

author = "Wakefield, {A. E.} and Keely, {S. P.} and Stringer, {J. R.} and Christensen, {C. B. V.} and Peter Ahrens and Peters, {S. E.} and Vivi Bille-Hansen and Jorsal, {Sven Erik Lind} and Henriksen, {S. A.} and O.P. Settnes",

year = "1997",

language = "English",

volume = "105",

pages = "317--321",

journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",

issn = "0903-4641",

publisher = "Wiley Online",

number = "4",

}

Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification. / Wakefield, A. E.; Keely, S. P.; Stringer, J. R.; Christensen, C. B. V.; Ahrens, Peter; Peters, S. E.; Bille-Hansen, Vivi; Jorsal, Sven Erik Lind; Henriksen, S. A.; Settnes, O.P.

In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 105, No. 4, 1997, p. 317-321.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification

AU - Wakefield, A. E.

AU - Keely, S. P.

AU - Stringer, J. R.

AU - Christensen, C. B. V.

AU - Ahrens, Peter

AU - Peters, S. E.

AU - Bille-Hansen, Vivi

AU - Jorsal, Sven Erik Lind

AU - Henriksen, S. A.

AU - Settnes, O.P.

PY - 1997

Y1 - 1997

N2 - DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.

AB - DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.

KW - nucleotide sequence

KW - Pneumocystis carinii

KW - DNA amplification

KW - pig

M3 - Journal article

VL - 105

SP - 317

EP - 321

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 4

ER -