Identification of Ononitol and O-methyl-scyllo-inositol in Pea Root Nodules

Leif Skøt, Helge Egsgaard

    Research output: Contribution to journalJournal articleResearchpeer-review


    Ononitol (4-O-methyl-myo-inositol) and O-methyl-scyllo-inositol were identified in pea (Pisum sativum L.) root nodules formed by twoRhizobium leguminosarum strains. Ononitol was the major soluble carbohydrate in nodules formed by strain 1045 while O-methyl-scyllo-inositol and two unidentified components were dominant in the carbohydrate pattern of the nodules formed by strain 1 a. The cyclitols were also present in the denodulated roots, but to a much smaller extent; in the above-ground plant parts only traces were found. The identification of ononitol and O-methyl-scyllo-inositol was established by gas chromatography and gas chromatography-mass spectrometry utilizing trimethylsilyl- and acetyl-derivatives.
    Original languageEnglish
    Issue number1
    Pages (from-to)32-36
    Publication statusPublished - 1984

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