Identification of novel candidate genes by exome sequencing in Tunisian familial male breast cancer patients

Wala Ben Kridis-Rejeb, Dorra Ben Ayed-Guerfali, Nihel Ammous-Boukhris, Wajdi Ayadi, Chamseddine Kifagi, Slim Charfi, Ines Saguem, Tahia Sellami-Boudawara, Jamel Daoud, Afef Khanfir, Raja Mokdad-Gargouri*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Male Breast Cancer (MBC) is a rare and aggressive disease that is associated with genetic factors. Mutations in BRCA1 and BRCA2 account for 10% of all MBC cases suggesting that other genetic factors are involved. The aim of the present study is to screen whole BRCA1 and BRCA2 exons using the Ampliseq BRCA panel in Tunisian MBC patients with family history. Furthermore, we performed exome sequencing using the TruSight One sequencing panel on an early onset BRCA negative patient. We showed that among the 6 MBC patients, only one (MBC-F1) harbored a novel frameshift mutation in exon 2 of the BRCA2 gene (c.17-20delAAGA, p.Lys6Xfs) resulting in a short BRCA2 protein of only 6 amino-acids. We selected 9 rare variants after applying several filter steps on the exome sequencing data. Among these variants, and based on their role in breast carcinogenesis, we retained 6 candidate genes (MSH5, DCC, ERBB3, NOTCH3, DIAPH1, and DNAH11). Further studies are needed to confirm the association of the selected genes with family MBC.
Original languageEnglish
JournalMolecular Biology Reports
Volume47
Issue number9
Pages (from-to)6507-6516
ISSN0301-4851
DOIs
Publication statusPublished - 2020

Keywords

  • BRCA1
  • BRCA2
  • Male breast cancer
  • Candidate genes
  • Next generation sequencing
  • Panel-based exome sequencing

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