Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum

Gianni Panagiotou, Emmanouil Papadakis, E. Topakas, Lisbeth Olsson, P. Christakopoulos

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    ATP-NADH kinase phosphorylates NADH to produce NADPH at the expense of ATP. The present study describes Fusarium oxysporum NADH kinase (ATP:NADH 2'-phosphotransferase, EC 2.7.1.86), a novel fungal enzyme capable of synthesizing NADPH using NADH as the preferred diphosphonicotinamide (diphosphopyridine) nucleotide donor. NADH kinase was highly purified (similar to 66-fold) and the enzyme was found to be a homodimeric with a subunit of M-r 72,000. Isoelectric focusing in the pH range of 3.0-9.5 of the purified NADH kinase yielded a pl value of about 5.6. The K-m values of NADH kinase for NADH and ATP were found to be 0.13 and 2.59 mM, respectively. Prediction of the secondary structure of the protein was performed in the PSIPRED server while modelling the three-dimensional (3D) structure was accomplished by the use of the HH 3D-structure prediction server. (C) 2008 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    JournalProcess Biochemistry
    Volume43
    Issue number10
    Pages (from-to)1114-1120
    ISSN1359-5113
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Dive into the research topics of 'Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum'. Together they form a unique fingerprint.

    Cite this