Identification of mustelid species: otter ( Lutra lutra ), American mink ( Mustela vison ) and polecat ( Mustela putorius ), by analysis of DNA from faecal samples

Michael Møller Hansen, Lene Jacobsen

Research output: Contribution to journalJournal articlepeer-review

Abstract

The abundance of mink, otter, and polecat may be inferred from records of their seats, and the feeding biology of the species is often studied by analysis of skeletal remains and other hard parts in faeces. However, in some situations it is difficult to distinguish between faeces from these three mustelid species. A method is described for assigning faeces to these three mustelid species, based on analysis of DNA extracted from their seats. Mustelid-specific primers were developed for PCR amplification of a part of the mitochondrial cytochrome b gene, and two restriction enzymes were found to detect species- specific sequence variation. Analysis of DNA from different faecal samples showed that the results were reproducible and that the approach provided an efficient method of species identification.
Original languageEnglish
JournalJournal of Zoology
Volume247
Issue number2
Pages (from-to)177-181
ISSN0952-8369
Publication statusPublished - 1999

Bibliographical note

J English Article CAMBRIDGE UNIV PRESS FEB 2 180BR NEW YORK Hansen MM Danish Inst Fisheries Res, Dept Inland Fisheries, Vejlsovej 39, DK-8600 Silkeborg, Denmark J ZOOL 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA

Fingerprint

Dive into the research topics of 'Identification of mustelid species: otter ( Lutra lutra ), American mink ( Mustela vison ) and polecat ( Mustela putorius ), by analysis of DNA from faecal samples'. Together they form a unique fingerprint.

Cite this