Identification of meat products by shotgun spectral matching

D. Ohana, H. Dalebout, R. J. Marissen, Tune Wulff, J. Bergquist, A. M. Deelder, M. Palmblad

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A new method, based on shotgun spectral matching of peptide tandem mass spectra, was successfully applied to the identification of different food species. The method was demonstrated to work on raw as well as processed samples from 16 mammalian and 10 bird species by counting spectral matches to spectral libraries in a reference database with one spectral library per species. A phylogenetic tree could also be constructed directly from the spectra. Nearly all samples could be correctly identified at the species level, and 100% at the genus level. The method does not use any genomic information and unlike targeted methods, no prior knowledge of genetic variation within a genus or species is necessary. (c) 2016 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalFood Chemistry
Volume203
Pages (from-to)28-34
Number of pages7
ISSN0308-8146
DOIs
Publication statusPublished - 2016

Keywords

  • Food products
  • Shotgun proteomics
  • Identification
  • Spectral libraries
  • Quantitation

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