Identification of cross-reactive human single-chain variable fragments against phospholipases A2 from Lachesis muta and Bothrops spp venoms

Lucas B. Campos, Manuela B. Pucca*, Luciano C. Silva, Gabriela Pessenda, Bruno A. Filardi, Felipe A. Cerni, Isadora S. Oliveira, Andreas H. Laustsen, Eliane C. Arantes, José E Barbosa

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A2 from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay.
Original languageEnglish
JournalToxicon
Volume184
Pages (from-to)116-121
ISSN0041-0101
DOIs
Publication statusPublished - 2020

Keywords

  • Antivenom
  • Monoclonal antibody
  • Lechesis muta
  • Bothrops spp
  • Cross-reactive antibodies
  • Phage display

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