Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza A virus strains in pigs

Massimiliano Baratelli, Lasse Eggers Pedersen, Ramona Trebbien, Lars Erik Larsen, Gregers Jungersen, Esther Blanco, Jens Nielsen, Maria Montoya

    Research output: Contribution to journalJournal articleResearchpeer-review

    101 Downloads (Pure)

    Abstract

    Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between 'avian-like' H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.
    Original languageEnglish
    JournalJournal of General Virology
    Volume98
    Issue number5
    Pages (from-to)895-899
    ISSN0022-1317
    DOIs
    Publication statusPublished - 2017

    Cite this