It is highly likely that DD-associated treponemes possess considerable antigenic variation, as cows exhibit a variable humoral response against different isolates of Treponema. Hence, combinations of antigens from multiple Treponema species should be used for the development of disease prevention measures. As treponemes from DD lesions are extremely difficult to culture, identification of these antigens is challenging. To circumvent this problem, we studied the in situ gene expression patterns of the microbiome in DD-affected skin lesions and the host antibody response directed at the site of infection. By metatranscriptomics we measured the in situ genome-wide transcriptome of the bacterial population in DD-affected skin lesions from 21 dairy cows. From the transcriptome data, we identified a panel of Treponema genes that were highly expressed in multiple animals, and we monitored the host immune response to these target genes using high-density peptide microarrays. By this approach, we identified a small group of antigenic proteins, which were expressed in the majority of the samples, and demonstrated antigenicity when screened against sera from infected animal. Future studies will show if these proteins represent candidates for the development of novel biomarkers or vaccines.
|Number of pages||1|
|Publication status||Published - 2016|
|Event||2nd International Symposium Alternatives to Antibiotics - Paris, France|
Duration: 12 Dec 2016 → 15 Dec 2016
Conference number: 2
|Conference||2nd International Symposium Alternatives to Antibiotics|
|Period||12/12/2016 → 15/12/2016|