A phosphoglucoisomerase locus (Pgi-2) segregates for four alleles in chromosome-doubled plants of the two ryegrasses, Lolium perenne and L. multiflorum. After separation by starch gel electrophoresis, all the possible 35 genotypes have been identified by means of the gene dosage effect on the isoenzyme bands. The Pgi-2 enzyme is a dimer and the zymogramme patterns show a maximum of seven bands, three of which are hybrid bands. An explanation of the complex variation in banding pattern is given. The existence of a fifth allele is shown and a possible effect of aneuploidy is discussed.
|Publication status||Published - 1980|