Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene

Lars Mølbak; Kirstine Klitgaard Schou; Tim Kåre Jensen; Marja Fossi; Mette Boye

doi:10.1128/JCM.01537-06

Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene

Lars Mølbak, Kirstine Klitgaard Schou, Tim Kåre Jensen, Marja Fossi, Mette Boye

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantii(T) (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	12
Pages (from-to)	4537-4540
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.01537-06
Publication status	Published - 2006

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title = "Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene",

abstract = "Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantii(T) (90.1%). The spirochete, here named {"}Candidatus Treponema suis,{"} was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.",

author = "Lars M{\o}lbak and Schou, {Kirstine Klitgaard} and Jensen, {Tim K{\aa}re} and Marja Fossi and Mette Boye",

year = "2006",

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language = "English",

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pages = "4537--4540",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology",

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Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene. / Mølbak, Lars; Schou, Kirstine Klitgaard; Jensen, Tim Kåre et al.
In: Journal of Clinical Microbiology, Vol. 44, No. 12, 2006, p. 4537-4540.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene

AU - Mølbak, Lars

AU - Schou, Kirstine Klitgaard

AU - Jensen, Tim Kåre

AU - Fossi, Marja

AU - Boye, Mette

PY - 2006

Y1 - 2006

N2 - Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantii(T) (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.

AB - Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantii(T) (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.

U2 - 10.1128/JCM.01537-06

DO - 10.1128/JCM.01537-06

M3 - Journal article

C2 - 17005743

SN - 0095-1137

VL - 44

SP - 4537

EP - 4540

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 12

ER -

Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene

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