Identification and typing of Vibrio anguillarum: A comparison of different methods.

B. Austin, M. Alsina, D. A. Austin, A. R. Blanch, F. Grimont, P. A. D. Grimont, J. Jofre, S. Koblavi, J. L. Larsen, Karl Pedersen, T. Tiainen, L. Verdonck, J. Swings

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Abstract

The majority (91%) of 260 isolates initially identified as Vibrio anguillarum, that were obtained from a wide range of hosts, habitats and geographical locations, were recovered in a single cluster based on the ribotype and were pathogenic to Atlantic salmon. A significant proportion of isolates (78% of the total) were allocated to 15 serogroups (O1 - O10 and five previously undescribed groups referred to as VaNT1, VaNT2, VaNT4, NaNT5 and VaNT7). A minority of isolates (6%) reacted with more than one antiserum or were self-agglutinating, and the remainder did not react with any of the antisera tested. Good correlation was noted between serogroups and lipopolysaccharide profiles, particularly with respect to isolates belonging to serogroups O1, O2 and O4 - O10. Plasmids were recognized in some serogroups, especially O1, which contained the 67 kb plasmid associated with virulence. However, the 19 profiles based on outer membrane protein patterns did not correspond to the results obtained with the other typing methods. Generally, the isolates were heterogeneous in their biochemical characteristics; 117 profiles were obtained with the API20E system, and 9 and 32 clusters recognised from the results of BIOLOG fingerprinting and Biotype-100 biotyping methods, respectively. Three dominant clusters were defined from fatty acid methyl esters profiles.
Original languageEnglish
JournalSystematic and Applied Microbiology
Volume18
Issue number2
Pages (from-to)285-302
Number of pages18
ISSN0723-2020
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • MICROBIOLOGY
  • BIOTECHNOLOGY
  • RIBOSOMAL-RNA
  • POLYACRYLAMIDE GELS
  • PLASMID
  • FISH
  • VIRULENCE
  • STRAINS
  • LISTONELLA
  • TAXONOMY
  • API-20E
  • ORDALII
  • VIBRIO
  • FISH ISOLATES
  • FISH PATHOGENS
  • IDENTIFICATION
  • TYPING METHODS
  • Vibrio
  • Fish isolates
  • Fish pathogens
  • Identification
  • Typing methods
  • ATLANTIC SALMON PATHOGEN
  • FATTY ACID METHYL ESTER PROFILE
  • LIPOPOLYSACCHARIDE PROFILE
  • MARICULTURE RELEVANCE
  • RIBOTYPE
  • SEROGROUPING
  • TYPING METHOD
  • VIRULENCE PLASMID
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Enterobacteriaceae
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Listonella anguillarum Vibrio anguillarum
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Osteichthyes
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 07510, Ecology: environmental biology - Oceanography and limnology
  • 07512, Ecology: environmental biology - Oceanography
  • 07516, Ecology: environmental biology - Wildlife management: aquatic
  • 10010, Comparative biochemistry
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 32000, Microbiological apparatus, methods and media
  • 34502, Immunology - General and methods
  • 36002, Medical and clinical microbiology - Bacteriology
  • 62510, Chordata: general and systematic - Pisces
  • Chemical Coordination and Homeostasis
  • Biochemistry and Molecular Biophysics
  • General Life Studies
  • Immune System
  • Infection
  • Methods and Techniques
  • Physiology
  • Systematics and Taxonomy

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