TY - JOUR
T1 - Identification and complete genome analysis of novel picornavirus in bovine in Japan
AU - Nagai, Makoto
AU - Omatsu, Tsutomu
AU - Aoki, Hiroshi
AU - Kaku, Yoshihiro
AU - Belsham, Graham
AU - Haga, Kei
AU - Naoi, Yuki
AU - Sano, Kaori
AU - Umetsu, Moeko
AU - Shiokawa, Mai
AU - Tsuchiaka, Shinobu
AU - Furuya, Tetsuya
AU - Okazaki, Sachiko
AU - Katayama, Yukie
AU - Oba, Mami
AU - Shirai, Junsuke
AU - Katayama, Kazuhiko
AU - Mizutani, Tetsuya
PY - 2015
Y1 - 2015
N2 - We identified novel viruses in feces from cattle with diarrhea collected in 2009 in
Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the
(near) complete sequences of the virus. Sequence analyses revealed that they had a
standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1
(VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They
are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group
1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9%
(P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses
and detailed genome characterization showed that they, together with the unclassified
Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding
regions. These viruses had conserved features (e.g. predicted protein cleavage sites,
presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they
have a common ancestor. Reverse-transcription-PCR assays, using specific primers
designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal
samples from cattle with diarrhea were positive, indicating the prevalence of these
picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further
studies are needed to investigate the pathogenic potential and etiological role of these
viruses in cattle.
AB - We identified novel viruses in feces from cattle with diarrhea collected in 2009 in
Hokkaido Prefecture, Japan, by using a metagenomics approach and determined the
(near) complete sequences of the virus. Sequence analyses revealed that they had a
standard picornavirus genome organization, i.e. 5' untranslated region (UTR) - L- P1
(VP4- VP3- VP2- VP1) - P2 (2A- 2B- 2C) - P3 (3A- 3B- 3C-3D) - 3'UTR- poly(A). They
are closely related to other unclassified Chinese picornaviruses; bat picornaviruses group
1-3, feline picornavirus, and canine picornavirus, sharing 45.4-51.4% (P1), 38.0-44.9%
(P2), and 49.6-53.3% (P3) amino acid identities, respectively. The phylogenetic analyses
and detailed genome characterization showed that they, together with the unclassified
Chinese picornaviruses, grouped as a cluster for the P1, 2C, 3CD and VP1 coding
regions. These viruses had conserved features (e.g. predicted protein cleavage sites,
presence of a leader protein, 2A, 2C, 3C, and 3D functional domains), suggesting they
have a common ancestor. Reverse-transcription-PCR assays, using specific primers
designed from the 5'UTR sequence of these viruses, showed that 23.0% (20/87) of fecal
samples from cattle with diarrhea were positive, indicating the prevalence of these
picornavirus in the Japanese cattle population in Hokkaido Prefecture. However, further
studies are needed to investigate the pathogenic potential and etiological role of these
viruses in cattle.
KW - Bovine
KW - Feces
KW - Japan
KW - Metagenomics
KW - Novel picornavirus
U2 - 10.1016/j.virusres.2015.08.001
DO - 10.1016/j.virusres.2015.08.001
M3 - Journal article
C2 - 26260333
VL - 210
SP - 205
EP - 212
JO - Virus Research
JF - Virus Research
SN - 0168-1702
ER -