Identification and characterization of protein cross-links induced by oxidative reactions

Per Hägglund*, Michele Mariotti, Michael J. Davies

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Introduction: Protein cross-links are common in biological systems and are generated both deliberately as a part of normal metabolism and also accidently as a result of exposure to external factors such as oxidation and glycation stresses. These cross-links can be both positive and negative for biological function, with high levels of inappropriate cross-links being associated with multiple human pathologies, as well as loss of protein structure and function in the food, agricultural, and pharmaceutical fields. Areas covered: This review covers recent advances in the detection and identification of protein cross-links arising from oxidation reactions, mediated by both radicals and two-electron oxidants. Information on both enzymatic and nonenzymatic cross-linking is reviewed, both where this is intentional, as part of normal metabolism, and accidental and a potential cause of disease. Expert commentary: The advantages and drawbacks of various methods available for the detection, identification, and quantification of these species are discussed, as well as some of the mechanisms and processes known to give rise to these species.

Original languageEnglish
JournalExpert Review of Proteomics
Volume15
Issue number8
Pages (from-to)665-681
ISSN1478-9450
DOIs
Publication statusPublished - 2018

Keywords

  • Cross-linking
  • Mass spectrometry
  • Protein oxidation

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