Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda; Clement Da Silva; Fatemeh Ahmadi; Allan Mcl Mowat; Hiroshi Ohno; Knut Kotarsky; William W. Agace

doi:10.1111/sji.13219

Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda, Clement Da Silva, Fatemeh Ahmadi, Allan Mcl Mowat, Hiroshi Ohno, Knut Kotarsky, William W. Agace^*

^*Corresponding author for this work

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Abstract

The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103⁺CD11b⁺ cDC in the murine small intestine (SI). GP2 expression was induced on CD103⁺CD11b⁺ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103⁺CD11b⁺ cDC (huLang-Cre.gp2^fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103⁺CD11b⁺ cDC is not required for intestinal immune homoeostasis.

Original language	English
Article number	e13219
Journal	Scandinavian Journal of Immunology
Volume	96
Issue number	5
Number of pages	12
ISSN	0300-9475
DOIs	https://doi.org/10.1111/sji.13219
Publication status	Published - 2022

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title = "Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells",

abstract = "The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.",

author = "Luda, {Katarzyna M.} and {Da Silva}, Clement and Fatemeh Ahmadi and Mowat, {Allan Mcl} and Hiroshi Ohno and Knut Kotarsky and Agace, {William W.}",

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AU - Luda, Katarzyna M.

AU - Da Silva, Clement

AU - Ahmadi, Fatemeh

AU - Mowat, Allan Mcl

AU - Ohno, Hiroshi

AU - Kotarsky, Knut

AU - Agace, William W.

PY - 2022

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N2 - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

AB - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

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JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

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Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

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