@inbook{38f80ae651eb4876bb7de27d68d5433d,
title = "Identification and characterization of antigen-specific t-cells in viral infections",
abstract = "Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.",
keywords = "CD8+ T-cells, Cytokine secretion assay, Flow cytometry, Rotavirus, SARS-CoV-2, T-cell epitopes, T-cell staining, Tetramers",
author = "Darya Trubach and Muleta, {Konjit Getachew} and Katharina Lahl and Saini, {Sunil Kumar}",
note = "Publisher Copyright: {\textcopyright} The Author(s).",
year = "2024",
doi = "10.1007/978-1-0716-3890-3_19",
language = "English",
isbn = "978-1-0716-3889-7",
volume = "2813",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "295--308",
booktitle = "Methods in Molecular Biology",
address = "United States",
}