Identification and characterization of antigen-specific t-cells in viral infections

Darya Trubach, Konjit Getachew Muleta, Katharina Lahl, Sunil Kumar Saini

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Volume2813
PublisherHumana Press
Publication date2024
Pages295-308
ISBN (Print)978-1-0716-3889-7
ISBN (Electronic)978-1-0716-3890-3
DOIs
Publication statusPublished - 2024
SeriesMethods in Molecular Biology
Volume2813
ISSN1064-3745

Keywords

  • CD8+ T-cells
  • Cytokine secretion assay
  • Flow cytometry
  • Rotavirus
  • SARS-CoV-2
  • T-cell epitopes
  • T-cell staining
  • Tetramers

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