Bifidobacterium species associated with breastfeeding produce aromatic lactic acids in the infant gut

Martin F. Laursen, Mikiyasu Sakanaka, Nicole von Burg, Urs Mörbe, Daniel Andersen, Janne Marie Moll, Ceyda T. Pekmez, Aymeric Rivollier, Kim F. Michaelsen, Christian Mølgaard, Mads Vendelbo Lind, Lars O. Dragsted, Takane Katayama, Henrik L. Frandsen, Anne Marie Vinggaard, Martin I. Bahl, Susanne Brix, William Agace, Tine R. Licht*, Henrik M. Roager*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Breastfeeding profoundly shapes the infant gut microbiota, which is critical for early life immune development, and the gut microbiota can impact host physiology in various ways, such as through the production of metabolites. However, few breastmilk-dependent microbial metabolites mediating host-microbiota interactions are currently known. Here, we demonstrate that breastmilk-promoted Bifidobacterium species convert aromatic amino acids (tryptophan, phenylalanine and tyrosine) into their respective aromatic lactic acids (indolelactic acid, phenyllactic acid and 4-hydroxyphenyllactic acid) via a previously unrecognized aromatic lactate dehydrogenase (ALDH). The ability of Bifidobacterium species to convert aromatic amino acids to their lactic acid derivatives was confirmed using monocolonized mice. Longitudinal profiling of the faecal microbiota composition and metabolome of Danish infants (n = 25), from birth until 6 months of age, showed that faecal concentrations of aromatic lactic acids are correlated positively with the abundance of human milk oligosaccharide-degrading Bifidobacterium species containing the ALDH, including Bifidobacterium longum, B. breve and B. bifidum. We further demonstrate that faecal concentrations of Bifidobacterium-derived indolelactic acid are associated with the capacity of these samples to activate in vitro the aryl hydrocarbon receptor (AhR), a receptor important for controlling intestinal homoeostasis and immune responses. Finally, we show that indolelactic acid modulates ex vivo immune responses of human CD4+ T cells and monocytes in a dose-dependent manner by acting as an agonist of both the AhR and hydroxycarboxylic acid receptor 3 (HCA3). Our findings reveal that breastmilk-promoted Bifidobacterium species produce aromatic lactic acids in the gut of infants and suggest that these microbial metabolites may impact immune function in early life.
Original languageEnglish
Article number1367–1382
JournalNature Microbiology
Volume30
Number of pages30
ISSN2058-5276
DOIs
Publication statusPublished - 2021

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