Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review



  • Author: Kolpen, Mette

    University of Copenhagen, Denmark

  • Author: Lerche, Christian J

    University of Copenhagen, Denmark

  • Author: Kragh, Kasper Nørskov

    University of Copenhagen, Denmark

  • Author: Sams, Thomas

    Biomedical Engineering, Department of Electrical Engineering, Technical University of Denmark, Ørsteds Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Koren, Klaus

    University of Copenhagen, Denmark

  • Author: Jensen, Anna S

    Technical University of Denmark, Denmark

  • Author: Line, Laura

    University of Copenhagen, Denmark

  • Author: Bjarnsholt, Thomas

    University of Copenhagen, Denmark

  • Author: Ciofu, Oana

    University of Copenhagen, Denmark

  • Author: Moser, Claus

    University of Copenhagen, Denmark

  • Author: Kühl, Michael

    University of Copenhagen, Denmark

  • Author: Høiby, Niels

    University of Copenhagen, Denmark

  • Author: Jensen, Peter Ø

    University of Copenhagen, Denmark

View graph of relations

Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of endobronchial antibiotic-tolerant biofilm subject to strong oxygen (O2) depletion due to the activity of surrounding polymorphonuclear leukocytes. The exact mechanisms affecting the antibiotic susceptibility of biofilms remain unclear, but accumulating evidence suggests that the efficacy of several bactericidal antibiotics is enhanced by stimulation of aerobic respiration of pathogens, while lack of O2 increases their tolerance. In fact, the bactericidal effect of several antibiotics depends on active aerobic metabolism activity and the endogenous formation of reactive O2 radicals (ROS). In this study we aimed to apply hyperbaric oxygen treatment (HBOT) in order to sensitize anoxic P. aeruginosa agarose-biofilms established to mimic situations with intense O2 consumption by the host response in the cystic fibrosis (CF) lung. Application of HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically relevant durations and was accompanied by indications of restored aerobic respiration, involvement of endogenous lethal oxidative stress and increased bacterial growth. The findings highlight that oxygenation by HBOT improves the bactericidal activity of ciprofloxacin on P. aeruginosa biofilm and suggest that bacterial biofilms is sensitized to antibiotics by supplying hyperbaric O2.
Original languageEnglish
Article numberAAC.01024-17
JournalAntimicrobial Agents and Chemotherapy
Issue number9
Number of pages28
Publication statusPublished - 2017

Bibliographical note

Copyright © 2017 Kolpen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

CitationsWeb of Science® Times Cited: No match on DOI
Download as:
Download as PDF
Select render style:
Download as HTML
Select render style:
Download as Word
Select render style:

Download statistics

No data available

ID: 136886756