Hydrophobic deep eutectic solvent as a novel, efficient aid for extraction of intracellular proteins from S. cerevisiae

T. Tjalsma*, Z. Su, M. Malankowska, M. Pinelo

*Corresponding author for this work

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

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Abstract

In comparison to other solvents employed in solid-liquid extraction, deep eutectic solvents (DESs) possess distinct characteristics that enhance their sustainability, including low volatility, low toxicity, and non-flammability. Besides these
aspects, extraction studies involving DESs are simpler than conventional methods, requiring minimal advanced equipment. A DES contains a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD), which can form hydrogen bonds upon mixing, thus reducing lattice energy and the melting point. Hydrophilic DESs are the majority of applied DESs in extraction studies, but in the last years an increase in hydrophobic DESs can be seen. However, there are still less extraction studies with primary metabolites than with the more stable secondary metabolites. Furthermore, distribution of substrates is uneven as fruits and leaves have been studied more than single cell cultures. As the fermentation industry is expanding, it is vital to develop sustainable downstream methods of intracellular compounds.

Therefore, the primary objective of the presented study is to assess the potential of hydrophobic DESs for intracellular protein extraction of S. cerevisiae. Ten hydrophobic DESs were synthesized from six different compounds. 0.5 wt% DES was mixed with S. cerevisiae in a buffer solution for 1 hour. Protein quantification showed that small amounts of hydrophobic DES act as an aid for protein extraction, of which decanoic acid: lidocaine 2:1 showed the highest result followed by L-menthol: oleic acid 1:1
Original languageEnglish
Publication date2023
Number of pages1
Publication statusPublished - 2023
EventRecent Advances in Fermentation Technology Conference - Naples Grande Beach Resort, Florida, United States
Duration: 29 Oct 20231 Nov 2023

Conference

ConferenceRecent Advances in Fermentation Technology Conference
LocationNaples Grande Beach Resort
Country/TerritoryUnited States
CityFlorida
Period29/10/202301/11/2023

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