Abstract
In comparison to other solvents employed in solid-liquid extraction, deep eutectic solvents (DESs) possess distinct characteristics that enhance their sustainability, including low volatility, low toxicity, and non-flammability. Besides these
aspects, extraction studies involving DESs are simpler than conventional methods, requiring minimal advanced equipment. A DES contains a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD), which can form hydrogen bonds upon mixing, thus reducing lattice energy and the melting point. Hydrophilic DESs are the majority of applied DESs in extraction studies, but in the last years an increase in hydrophobic DESs can be seen. However, there are still less extraction studies with primary metabolites than with the more stable secondary metabolites. Furthermore, distribution of substrates is uneven as fruits and leaves have been studied more than single cell cultures. As the fermentation industry is expanding, it is vital to develop sustainable downstream methods of intracellular compounds.
Therefore, the primary objective of the presented study is to assess the potential of hydrophobic DESs for intracellular protein extraction of S. cerevisiae. Ten hydrophobic DESs were synthesized from six different compounds. 0.5 wt% DES was mixed with S. cerevisiae in a buffer solution for 1 hour. Protein quantification showed that small amounts of hydrophobic DES act as an aid for protein extraction, of which decanoic acid: lidocaine 2:1 showed the highest result followed by L-menthol: oleic acid 1:1
aspects, extraction studies involving DESs are simpler than conventional methods, requiring minimal advanced equipment. A DES contains a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD), which can form hydrogen bonds upon mixing, thus reducing lattice energy and the melting point. Hydrophilic DESs are the majority of applied DESs in extraction studies, but in the last years an increase in hydrophobic DESs can be seen. However, there are still less extraction studies with primary metabolites than with the more stable secondary metabolites. Furthermore, distribution of substrates is uneven as fruits and leaves have been studied more than single cell cultures. As the fermentation industry is expanding, it is vital to develop sustainable downstream methods of intracellular compounds.
Therefore, the primary objective of the presented study is to assess the potential of hydrophobic DESs for intracellular protein extraction of S. cerevisiae. Ten hydrophobic DESs were synthesized from six different compounds. 0.5 wt% DES was mixed with S. cerevisiae in a buffer solution for 1 hour. Protein quantification showed that small amounts of hydrophobic DES act as an aid for protein extraction, of which decanoic acid: lidocaine 2:1 showed the highest result followed by L-menthol: oleic acid 1:1
| Original language | English |
|---|---|
| Publication date | 2023 |
| Number of pages | 1 |
| Publication status | Published - 2023 |
| Event | Recent Advances in Fermentation Technology Conference - Naples Grande Beach Resort, Florida, United States Duration: 29 Oct 2023 → 1 Nov 2023 |
Conference
| Conference | Recent Advances in Fermentation Technology Conference |
|---|---|
| Location | Naples Grande Beach Resort |
| Country/Territory | United States |
| City | Florida |
| Period | 29/10/2023 → 01/11/2023 |