Hydrolysis of Nothogenia erinacea xylan by xylanases from families 10 and 11

W. Nerinckx, A. Broberg, Jens Øllgaard Duus, P. Ntarima, LAS Parolis, H. Parolis, M. Claeyssens

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The structures of several enzymatic hydrolysis products of Nothogenia erinacea seaweed xylan, a linear homopolymer with mixed ss-(1 --> 3)/ss-(1 --> 4) linkages, were analysed by physicochemical and biochemical techniques. With the glycoside hydrolase family 10 ss-(1 --> 4)-xylanase from Cryptococcus adeliae, hydrolysis proceeds to a final mixture of products containing a mixed linkage-type triose as a major compound, whereas with the family I I xylanase from Thermomyces lanuginosus this is a mixed linkage tetraose. The Cryptococcus xylanase is shown to be capable of also catalysing the hydrolysis of ss-(1 --> 3) linkages, that is this of a mixed type tetraose intermediary formed, in accordance with the broader substrate specificity of family 10 enzymes. From a partial degradation experiment with the T lanuginosus xylanase, a series of higher mixed oligosaccharides were isolated and identified. The observed oligosaccharide intermediates and splicing pattern indicate an irregular ss-(1 --> 3)/ss-(1 --> 4) linkage distribution within the linear D-xylose polymer. Similar results were obtained with rhodymenan, the seaweed xylan from Palmares palmata . (C) 2004 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCARBOHYDRATE RESEARCH
Volume339
Issue number6
Pages (from-to)1047-1060
ISSN0008-6215
DOIs
Publication statusPublished - 2004
Externally publishedYes

Keywords

  • Ascomycota
  • Carbohydrate Sequence
  • Cryptococcus
  • Endo-1,4-beta Xylanases
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides
  • Polysaccharides
  • Protons
  • Rhodophyta
  • Seaweed
  • Substrate Specificity
  • Time Factors
  • Trifluoroacetic Acid
  • Xylans
  • E5R8Z4G708 Trifluoroacetic Acid
  • EC 3.2.1.8 Endo-1,4-beta Xylanases
  • Biochemistry
  • Degradation
  • Enzymes
  • Homopolymerization
  • Molecular structure
  • Carbohydrates
  • fungal enzyme
  • glycosidase
  • oligosaccharide
  • polymer
  • rhodymenan
  • triose
  • unclassified drug
  • xylan
  • xylan endo 1,3 beta xylosidase
  • article
  • catalysis
  • chemical structure
  • Cryptococcus adeliae
  • enzyme degradation
  • enzyme specificity
  • fungal strain
  • hydrolysis
  • Nothogenia erinacea
  • Palmares palmata
  • physical chemistry
  • priority journal
  • protein family
  • Rhodymenan palmata
  • seaweed
  • thermomyces lanuginosus
  • Algae, Red
  • algae
  • Cryptococcus adeliensis
  • Erinacea
  • Thermomyces
  • Thermomyces lanuginosus
  • Enzymatic hydrolysis
  • Partial degradation
  • Physicochemical techniques
  • DP, degree of polymerisation
  • HPAEC-PAD, High performance anion exchange chromatography with pulsed amperometric detection
  • Rhodymenan
  • Xylanases
  • Xylooligosaccharides
  • GLYCOSIDASES
  • T
  • BIOCHEMISTRY
  • CHEMISTRY,
  • CATALYTIC PROPERTIES
  • PURIFICATION
  • FAMILIES
  • xylanases
  • xylooligosaccharides
  • Algae Plantae (Algae, Microorganisms, Nonvascular Plants, Plants) - Rhodophyta [14700] Nothogenia erinacea species
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Cryptococcus adeliae species Thermomyces lamuginosus species
  • D-xylose polymer
  • rhodymenan 9049-36-9
  • xylan 9014-63-5 hydrolysis
  • xylanases 9025-55-2Q, 9025-57-4Q, 37278-89-0Q family 10, family 11
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 51518, Plant physiology - Enzymes
  • Biochemistry and Molecular Biophysics
  • Enzymology

Cite this

Nerinckx, W., Broberg, A., Duus, J. Ø., Ntarima, P., Parolis, LAS., Parolis, H., & Claeyssens, M. (2004). Hydrolysis of Nothogenia erinacea xylan by xylanases from families 10 and 11. CARBOHYDRATE RESEARCH, 339(6), 1047-1060. https://doi.org/10.1016/j.carres.2004.02.017