Hydrogenotrophic methanogens are the key for a successful bioaugmentation to alleviate ammonia inhibition in thermophilic anaerobic digesters

Hailin Tian, Miao Yan, Laura Treu, Irini Angelidaki, Ioannis Fotidis*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bioaugmentation to alleviate ammonia inhibition under thermophilic anaerobic digestion has never been reported, as well as the working mechanism that allows a fast and successful bioaugmentation. Thus two bioaugmentation inocula (an enriched culture, and a mixed culture composed 50/50 by Methanoculleus thermophilus and the enriched culture) on the recovery of ammonia-inhibited thermophilic continuous reactors was assessed. The results showed that bioaugmentation improved methane yield by 11–13% and decreased the volatile fatty acids (VFA) by 45–52% compared to the control reactor (abiotic augmentation). Moreover, the importance of hydrogenotrophic methanogens to a fast and successful bioaugmentation was recognized. Specifically, the instant hydrogen partial pressure reduction by the bioaugmented hydrogenotroph created thermodynamically favourable conditions for the acetate oxidation process and consequently, the catabolism of other VFA. High-throughput sequencing results strengthened this explanation by showing that the bioaugmented M. thermophilus stimulated the growth of syntrophic acetate oxidising bacterium Thermacetogenium phaeum, immediately after bioaugmentation.

Original languageEnglish
Article number122070
JournalBioresource Technology
Volume293
Number of pages9
ISSN0960-8524
DOIs
Publication statusPublished - 2019

Keywords

  • Ammonia-tolerant consortium
  • Biogas
  • Methanoculleus thermophilus
  • Methanosarcina thermophila
  • Microbial community

Cite this

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title = "Hydrogenotrophic methanogens are the key for a successful bioaugmentation to alleviate ammonia inhibition in thermophilic anaerobic digesters",
abstract = "Bioaugmentation to alleviate ammonia inhibition under thermophilic anaerobic digestion has never been reported, as well as the working mechanism that allows a fast and successful bioaugmentation. Thus two bioaugmentation inocula (an enriched culture, and a mixed culture composed 50/50 by Methanoculleus thermophilus and the enriched culture) on the recovery of ammonia-inhibited thermophilic continuous reactors was assessed. The results showed that bioaugmentation improved methane yield by 11–13{\%} and decreased the volatile fatty acids (VFA) by 45–52{\%} compared to the control reactor (abiotic augmentation). Moreover, the importance of hydrogenotrophic methanogens to a fast and successful bioaugmentation was recognized. Specifically, the instant hydrogen partial pressure reduction by the bioaugmented hydrogenotroph created thermodynamically favourable conditions for the acetate oxidation process and consequently, the catabolism of other VFA. High-throughput sequencing results strengthened this explanation by showing that the bioaugmented M. thermophilus stimulated the growth of syntrophic acetate oxidising bacterium Thermacetogenium phaeum, immediately after bioaugmentation.",
keywords = "Ammonia-tolerant consortium, Biogas, Methanoculleus thermophilus, Methanosarcina thermophila, Microbial community",
author = "Hailin Tian and Miao Yan and Laura Treu and Irini Angelidaki and Ioannis Fotidis",
year = "2019",
doi = "10.1016/j.biortech.2019.122070",
language = "English",
volume = "293",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Hydrogenotrophic methanogens are the key for a successful bioaugmentation to alleviate ammonia inhibition in thermophilic anaerobic digesters. / Tian, Hailin; Yan, Miao; Treu, Laura; Angelidaki, Irini; Fotidis, Ioannis .

In: Bioresource Technology, Vol. 293, 122070, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Hydrogenotrophic methanogens are the key for a successful bioaugmentation to alleviate ammonia inhibition in thermophilic anaerobic digesters

AU - Tian, Hailin

AU - Yan, Miao

AU - Treu, Laura

AU - Angelidaki, Irini

AU - Fotidis, Ioannis

PY - 2019

Y1 - 2019

N2 - Bioaugmentation to alleviate ammonia inhibition under thermophilic anaerobic digestion has never been reported, as well as the working mechanism that allows a fast and successful bioaugmentation. Thus two bioaugmentation inocula (an enriched culture, and a mixed culture composed 50/50 by Methanoculleus thermophilus and the enriched culture) on the recovery of ammonia-inhibited thermophilic continuous reactors was assessed. The results showed that bioaugmentation improved methane yield by 11–13% and decreased the volatile fatty acids (VFA) by 45–52% compared to the control reactor (abiotic augmentation). Moreover, the importance of hydrogenotrophic methanogens to a fast and successful bioaugmentation was recognized. Specifically, the instant hydrogen partial pressure reduction by the bioaugmented hydrogenotroph created thermodynamically favourable conditions for the acetate oxidation process and consequently, the catabolism of other VFA. High-throughput sequencing results strengthened this explanation by showing that the bioaugmented M. thermophilus stimulated the growth of syntrophic acetate oxidising bacterium Thermacetogenium phaeum, immediately after bioaugmentation.

AB - Bioaugmentation to alleviate ammonia inhibition under thermophilic anaerobic digestion has never been reported, as well as the working mechanism that allows a fast and successful bioaugmentation. Thus two bioaugmentation inocula (an enriched culture, and a mixed culture composed 50/50 by Methanoculleus thermophilus and the enriched culture) on the recovery of ammonia-inhibited thermophilic continuous reactors was assessed. The results showed that bioaugmentation improved methane yield by 11–13% and decreased the volatile fatty acids (VFA) by 45–52% compared to the control reactor (abiotic augmentation). Moreover, the importance of hydrogenotrophic methanogens to a fast and successful bioaugmentation was recognized. Specifically, the instant hydrogen partial pressure reduction by the bioaugmented hydrogenotroph created thermodynamically favourable conditions for the acetate oxidation process and consequently, the catabolism of other VFA. High-throughput sequencing results strengthened this explanation by showing that the bioaugmented M. thermophilus stimulated the growth of syntrophic acetate oxidising bacterium Thermacetogenium phaeum, immediately after bioaugmentation.

KW - Ammonia-tolerant consortium

KW - Biogas

KW - Methanoculleus thermophilus

KW - Methanosarcina thermophila

KW - Microbial community

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ER -