Hydrodynamic impact of particle shape in slurry packed liquid chromatography columns

F. Lottes, W. Arlt, M. Minceva, Erling Halfdan Stenby

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We report on a series of flow velocity and efficiency profiles, which were measured across the cross section of preparative chromatographic columns packed with different stationary phase materials using computed tomography. It is shown that this non-invasive technique is very useful for visualization of the inner part of a packed column and measurement of the spatial resolved column packing properties. For evaluation of the influence of the particle shape on the velocity distribution and column performance, irregular and spherical reversed phases were studied in detail. The results showed a decreasing velocity towards the column wall most certainly due to a lower permeability. This effect was much less pronounced in the case of spherical particles, indicating a more homogenous packing structure. The influence of the column packing pressure, as a possible measure for improvement of the packing homogeneity was also studied. It was shown that under the same packing conditions spherical particles always lead to a more homogeneous packing. The overall results of this work contribute to the origin of the fact that spherical material is superior to irregular one from the hydrodynamic point of view.
Original languageEnglish
JournalJournal of Chromatography A
Volume1216
Issue number30
Pages (from-to)5687-5695
ISSN0021-9673
DOIs
Publication statusPublished - 2009

Keywords

  • Efficiency
  • Slurry packing
  • Liquid chromatography
  • Computed tomography
  • Particle shape

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