Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Bernadeth F.  Ticar; Zuliyati  Rohmah; Solange Ines Mussatto; Jae-Min  Lim; Seongha  Park; Byeong-Dae  Choi

doi:10.1016/j.carbpol.2016.12.065

Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Bernadeth F. Ticar
, Zuliyati Rohmah
, Solange Ines Mussatto
, Jae-Min Lim
, Seongha Park
, Byeong-Dae Choi

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.

Original language	English
Journal	Carbohydrate Polymers
Volume	161
Pages (from-to)	15-20
ISSN	0144-8617
DOIs	https://doi.org/10.1016/j.carbpol.2016.12.065
Publication status	Published - 2017

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title = "Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs",

abstract = "Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2\% sulfated glycans; 34.5, 38.2, and 45.0\% uronic acids; and 23.5, 19.0, and 7.5\% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.",

author = "Ticar, \{Bernadeth F.\} and Zuliyati Rohmah and Mussatto, \{Solange Ines\} and Jae-Min Lim and Seongha Park and Byeong-Dae Choi",

year = "2017",

doi = "10.1016/j.carbpol.2016.12.065",

language = "English",

volume = "161",

pages = "15--20",

journal = "Carbohydrate Polymers",

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TY - JOUR

T1 - Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

AU - Ticar, Bernadeth F.

AU - Rohmah, Zuliyati

AU - Mussatto, Solange Ines

AU - Lim, Jae-Min

AU - Park, Seongha

AU - Choi, Byeong-Dae

PY - 2017

Y1 - 2017

N2 - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.

AB - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.

U2 - 10.1016/j.carbpol.2016.12.065

DO - 10.1016/j.carbpol.2016.12.065

M3 - Journal article

C2 - 28189224

SN - 0144-8617

VL - 161

SP - 15

EP - 20

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Abstract

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