TY - JOUR
T1 - Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs
AU - Ticar, Bernadeth F.
AU - Rohmah, Zuliyati
AU - Mussatto, Solange Ines
AU - Lim, Jae-Min
AU - Park, Seongha
AU - Choi, Byeong-Dae
PY - 2017
Y1 - 2017
N2 - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.
AB - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.
U2 - 10.1016/j.carbpol.2016.12.065
DO - 10.1016/j.carbpol.2016.12.065
M3 - Journal article
C2 - 28189224
SN - 0144-8617
VL - 161
SP - 15
EP - 20
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -