Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.
Ticar, B. F., Rohmah, Z., Mussatto, S. I., Lim, J-M., Park, S., & Choi, B-D. (2017). Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs. Carbohydrate Polymers, 161, 15-20. https://doi.org/10.1016/j.carbpol.2016.12.065