With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.
|Publication status||Published - 2017|
|Event||19th European Carbohydrate Symposium - CCIB, Barcelona, Spain|
Duration: 2 Jul 2017 → 6 Jul 2017
Conference number: 19
|Conference||19th European Carbohydrate Symposium|
|Period||02/07/2017 → 06/07/2017|
Hansen, A. H., Amann, T., Kol, S., & Kildegaard, H. F. (2017). Humanizing recombinant glycoproteins from Chinese hamster ovary cells. Abstract from 19th European Carbohydrate Symposium, Barcelona, Spain.