Humanizing recombinant glycoproteins from Chinese hamster ovary cells

Anders Holmgaard Hansen, Thomas Amann, Stefan Kol, Helene Faustrup Kildegaard

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Abstract

With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.
Original languageEnglish
Publication date2017
Publication statusPublished - 2017
Event19th European Carbohydrate Symposium - CCIB, Barcelona, Spain
Duration: 2 Jul 20176 Jul 2017
Conference number: 19

Conference

Conference19th European Carbohydrate Symposium
Number19
LocationCCIB
CountrySpain
CityBarcelona
Period02/07/201706/07/2017

Cite this

Hansen, A. H., Amann, T., Kol, S., & Kildegaard, H. F. (2017). Humanizing recombinant glycoproteins from Chinese hamster ovary cells. Abstract from 19th European Carbohydrate Symposium, Barcelona, Spain.