Humanizing recombinant glycoproteins from Chinese hamster ovary cells

Anders Holmgaard Hansen; Thomas Amann; Stefan Kol; Helene Faustrup Kildegaard

Humanizing recombinant glycoproteins from Chinese hamster ovary cells

Anders Holmgaard Hansen, Thomas Amann, Stefan Kol, Helene Faustrup Kildegaard

Novo Nordisk Foundation Center for Biosustainability

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Abstract

With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.

Original language	English
Publication date	2017
Publication status	Published - 2017
Event	19th European Carbohydrate Symposium - CCIB, Barcelona, Spain Duration: 2 Jul 2017 → 6 Jul 2017 Conference number: 19

Conference

Conference	19th European Carbohydrate Symposium
Number	19
Location	CCIB
Country/Territory	Spain
City	Barcelona
Period	02/07/2017 → 06/07/2017

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title = "Humanizing recombinant glycoproteins from Chinese hamster ovary cells",

abstract = "With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.",

author = "Hansen, {Anders Holmgaard} and Thomas Amann and Stefan Kol and Kildegaard, {Helene Faustrup}",

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note = "19th European Carbohydrate Symposium, EUROCARB ; Conference date: 02-07-2017 Through 06-07-2017",

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T1 - Humanizing recombinant glycoproteins from Chinese hamster ovary cells

AU - Hansen, Anders Holmgaard

AU - Amann, Thomas

AU - Kol, Stefan

AU - Kildegaard, Helene Faustrup

N1 - Conference code: 19

PY - 2017

Y1 - 2017

N2 - With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.

AB - With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese hamster ovary (CHO) cells are making a very heterogeneous mixture of NGlycans. We speculate that the CHO pattern of N-Glycans would affect half-life and/or efficacy of the glycoprotein in the bloodstream making it unsuitable for human intravenous use, whereas our humanized version would be identical to the native human glycoprotein.

M3 - Conference abstract for conference

T2 - 19th European Carbohydrate Symposium

Y2 - 2 July 2017 through 6 July 2017

ER -

Humanizing recombinant glycoproteins from Chinese hamster ovary cells

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