Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction

Jens Christian Brasen; Anita Lunding; Lars Folke Olsen

doi:10.1016/j.abb.2004.07.019

Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction

Jens Christian Brasen, Anita Lunding, Lars Folke Olsen

University of Southern Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We have studied the peroxidase–oxidase reaction catalyzed by human myeloperoxidase in an open system where both sub- strates—molecular oxygen and NADH—are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental find- ings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils

Original language	English
Journal	Archives of Biochemistry and Biophysics
Volume	431
Pages (from-to)	55-62
ISSN	0003-9861
DOIs	https://doi.org/10.1016/j.abb.2004.07.019
Publication status	Published - 2004
Externally published	Yes

Access to Document

10.1016/j.abb.2004.07.019

OpenUrl availability

Full text

Cite this

@article{af25159a8fc941678f4d5fd9f03a7c8c,

title = "Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction",

abstract = "We have studied the peroxidase–oxidase reaction catalyzed by human myeloperoxidase in an open system where both sub- strates—molecular oxygen and NADH—are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental find- ings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils",

author = "Brasen, {Jens Christian} and Anita Lunding and Olsen, {Lars Folke}",

year = "2004",

doi = "10.1016/j.abb.2004.07.019",

language = "English",

volume = "431",

pages = "55--62",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press",

}

TY - JOUR

T1 - Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction

AU - Brasen, Jens Christian

AU - Lunding, Anita

AU - Olsen, Lars Folke

PY - 2004

Y1 - 2004

N2 - We have studied the peroxidase–oxidase reaction catalyzed by human myeloperoxidase in an open system where both sub- strates—molecular oxygen and NADH—are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental find- ings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils

AB - We have studied the peroxidase–oxidase reaction catalyzed by human myeloperoxidase in an open system where both sub- strates—molecular oxygen and NADH—are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental find- ings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils

U2 - 10.1016/j.abb.2004.07.019

DO - 10.1016/j.abb.2004.07.019

M3 - Journal article

SN - 0003-9861

VL - 431

SP - 55

EP - 62

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

ER -

Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction

Abstract

Access to Document

OpenUrl availability

Fingerprint

Cite this