Human myeloperoxidase catalyzes an oscillating peroxidase-oxidase reaction

Jens Christian Brasen, Anita Lunding, Lars Folke Olsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We have studied the peroxidase–oxidase reaction catalyzed by human myeloperoxidase in an open system where both sub- strates—molecular oxygen and NADH—are supplied continuously to the reaction mixture. The reaction shows oscillatory kinetics at pH values around 5, provided that the reaction medium in addition to the enzyme and the substrates also contains an aromatic electron mediator such as melatonin or 4-hydroxybenzoic acid and chloride ions at concentrations >1mM. The experimental find- ings can be simulated by a detailed model of the reaction. The results are important for our understanding of oxidant production in neutrophils
Original languageEnglish
JournalArchives of Biochemistry and Biophysics
Volume431
Pages (from-to)55-62
ISSN0003-9861
DOIs
Publication statusPublished - 2004
Externally publishedYes

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