How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox

Jacky L. Snoep; Peter Ruhdal Jensen; Philip Groeneveld; Douwe Molenaar; Boris N. Kholodenko; Hans V. Westerhoff

How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox

Jacky L. Snoep, Peter Ruhdal Jensen, Philip Groeneveld, Douwe Molenaar, Boris N. Kholodenko, Hans V. Westerhoff

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. this analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.

Original language	English
Journal	Biochemistry and Molecular Biology International
Volume	33
Issue number	5
Pages (from-to)	1023-1032
Number of pages	10
ISSN	1039-9712
Publication status	Published - 1994

Keywords

Bacteria
Biomass
Culture Media
Fungi
Kinetics
Metabolism
Models, Biological
ANALYTICAL METHOD
GROWTH LIMITING SUBSTRATE ANALYSIS
MICROBIAL PHYSIOLOGY
Microorganisms (Microorganisms) - Microorganisms [01000] microorganisms
10060, Biochemistry studies - General
13002, Metabolism - General metabolism and metabolic pathways
32000, Microbiological apparatus, methods and media
32500, Tissue culture, apparatus, methods and media
Methods and Techniques

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title = "How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox",

abstract = "The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. this analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.",

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author = "Snoep, {Jacky L.} and Jensen, {Peter Ruhdal} and Philip Groeneveld and Douwe Molenaar and Kholodenko, {Boris N.} and Westerhoff, {Hans V.}",

year = "1994",

language = "English",

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journal = "Biochemistry and Molecular Biology International",

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TY - JOUR

T1 - How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox

AU - Snoep, Jacky L.

AU - Jensen, Peter Ruhdal

AU - Groeneveld, Philip

AU - Molenaar, Douwe

AU - Kholodenko, Boris N.

AU - Westerhoff, Hans V.

PY - 1994

Y1 - 1994

N2 - The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. this analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.

AB - The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. this analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.

KW - Bacteria

KW - Biomass

KW - Culture Media

KW - Fungi

KW - Kinetics

KW - Metabolism

KW - Models, Biological

KW - ANALYTICAL METHOD

KW - GROWTH LIMITING SUBSTRATE ANALYSIS

KW - MICROBIAL PHYSIOLOGY

KW - Microorganisms (Microorganisms) - Microorganisms [01000] microorganisms

KW - 10060, Biochemistry studies - General

KW - 13002, Metabolism - General metabolism and metabolic pathways

KW - 32000, Microbiological apparatus, methods and media

KW - 32500, Tissue culture, apparatus, methods and media

KW - Methods and Techniques

M3 - Journal article

SN - 1039-9712

VL - 33

SP - 1023

EP - 1032

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

IS - 5

ER -

How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox

Abstract

Keywords

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