How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox

Jacky L. Snoep, Peter Ruhdal Jensen, Philip Groeneveld, Douwe Molenaar, Boris N. Kholodenko, Hans V. Westerhoff

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. this analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.
    Original languageEnglish
    JournalBiochemistry and Molecular Biology International
    Volume33
    Issue number5
    Pages (from-to)1023-1032
    Number of pages10
    ISSN1039-9712
    Publication statusPublished - 1994

    Keywords

    • Bacteria
    • Biomass
    • Culture Media
    • Fungi
    • Kinetics
    • Metabolism
    • Models, Biological
    • ANALYTICAL METHOD
    • GROWTH LIMITING SUBSTRATE ANALYSIS
    • MICROBIAL PHYSIOLOGY
    • Microorganisms (Microorganisms) - Microorganisms [01000] microorganisms
    • 10060, Biochemistry studies - General
    • 13002, Metabolism - General metabolism and metabolic pathways
    • 32000, Microbiological apparatus, methods and media
    • 32500, Tissue culture, apparatus, methods and media
    • Methods and Techniques

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