Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System

Angela Victoria Holguín*, Pablo Cárdenas, Catalina Prada-Peñaranda, Laura Rabelo Leite, Camila Buitrago, Viviana Clavijo, Guilherme Oliveira, Pimlapas Leekitcharoenphon, Frank Møller Aarestrup, Martha J. Vives

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.

Original languageEnglish
Article number188
JournalViruses
Volume11
Issue number2
Number of pages22
ISSN1999-4915
DOIs
Publication statusPublished - 2019

Keywords

  • antibiotics
  • bacteria-phage coevolution
  • bacterial resistance
  • phage-therapy
  • Salmonella Enteritidis

Cite this

Holguín, A. V., Cárdenas, P., Prada-Peñaranda, C., Rabelo Leite, L., Buitrago, C., Clavijo, V., ... Vives, M. J. (2019). Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses, 11(2), [188]. https://doi.org/10.3390/v11020188
Holguín, Angela Victoria ; Cárdenas, Pablo ; Prada-Peñaranda, Catalina ; Rabelo Leite, Laura ; Buitrago, Camila ; Clavijo, Viviana ; Oliveira, Guilherme ; Leekitcharoenphon, Pimlapas ; Møller Aarestrup, Frank ; Vives, Martha J. / Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. In: Viruses. 2019 ; Vol. 11, No. 2.
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abstract = "Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.",
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Holguín, AV, Cárdenas, P, Prada-Peñaranda, C, Rabelo Leite, L, Buitrago, C, Clavijo, V, Oliveira, G, Leekitcharoenphon, P, Møller Aarestrup, F & Vives, MJ 2019, 'Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System', Viruses, vol. 11, no. 2, 188. https://doi.org/10.3390/v11020188

Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. / Holguín, Angela Victoria; Cárdenas, Pablo; Prada-Peñaranda, Catalina; Rabelo Leite, Laura; Buitrago, Camila; Clavijo, Viviana; Oliveira, Guilherme; Leekitcharoenphon, Pimlapas; Møller Aarestrup, Frank; Vives, Martha J.

In: Viruses, Vol. 11, No. 2, 188, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System

AU - Holguín, Angela Victoria

AU - Cárdenas, Pablo

AU - Prada-Peñaranda, Catalina

AU - Rabelo Leite, Laura

AU - Buitrago, Camila

AU - Clavijo, Viviana

AU - Oliveira, Guilherme

AU - Leekitcharoenphon, Pimlapas

AU - Møller Aarestrup, Frank

AU - Vives, Martha J.

PY - 2019

Y1 - 2019

N2 - Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.

AB - Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.

KW - antibiotics

KW - bacteria-phage coevolution

KW - bacterial resistance

KW - phage-therapy

KW - Salmonella Enteritidis

U2 - 10.3390/v11020188

DO - 10.3390/v11020188

M3 - Journal article

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 2

M1 - 188

ER -

Holguín AV, Cárdenas P, Prada-Peñaranda C, Rabelo Leite L, Buitrago C, Clavijo V et al. Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System. Viruses. 2019;11(2). 188. https://doi.org/10.3390/v11020188