TY - JOUR
T1 - Horseradish peroxidase immobilised onto electrospun fibres and its application in decolourisation of dyes from model sea water
AU - Jankowska, Katarzyna
AU - Zdarta, Jakub
AU - Grzywaczyk, Adam
AU - Degórska, Oliwia
AU - Kijeńska-Gawrońska, Ewa
AU - Pinelo, Manuel
AU - Jesionowski, Teofil
PY - 2021
Y1 - 2021
N2 - Despite many studies concerning dyes decolourisation, we propose a novel, cheap, eco-friendly and sustainable solution for highly effective dyes removal based on electrospun materials and immobilised enzyme. Our study provides proof-of-concept for the immobilisation of horseradish peroxidase (HRP) on polyamide 6 electrospun fibres by adsorption and covalent binding, and application for dyes decolourisation from imitating polluted sea waters. The highest relative activity of immobilised HRP was obtained after immobilisation performed at 50 °C and pH 7 for 60 min for adsorption method, as well as using 3% of glutaraldehyde concentration, 70 min of process time, 60 °C and pH 7 for covalent binding. Biocatalytic systems produced were found to possess high catalytic properties and were used for decolourisation of Reactive Black 5 and Malachite Green textile dyes from solutions imitating polluted sea waters, that is a novel approach for removal of pollutants from aqueous solutions. The highest decolourisation efficiencies of dyes were obtained after 60 min of treatment at pH 7 and temperature 25 °C and reached over 70%. In the future, the presented approach based on use of enzymes immobilised in decolourisation processes, might play significant role in industrial removal of dyes.
AB - Despite many studies concerning dyes decolourisation, we propose a novel, cheap, eco-friendly and sustainable solution for highly effective dyes removal based on electrospun materials and immobilised enzyme. Our study provides proof-of-concept for the immobilisation of horseradish peroxidase (HRP) on polyamide 6 electrospun fibres by adsorption and covalent binding, and application for dyes decolourisation from imitating polluted sea waters. The highest relative activity of immobilised HRP was obtained after immobilisation performed at 50 °C and pH 7 for 60 min for adsorption method, as well as using 3% of glutaraldehyde concentration, 70 min of process time, 60 °C and pH 7 for covalent binding. Biocatalytic systems produced were found to possess high catalytic properties and were used for decolourisation of Reactive Black 5 and Malachite Green textile dyes from solutions imitating polluted sea waters, that is a novel approach for removal of pollutants from aqueous solutions. The highest decolourisation efficiencies of dyes were obtained after 60 min of treatment at pH 7 and temperature 25 °C and reached over 70%. In the future, the presented approach based on use of enzymes immobilised in decolourisation processes, might play significant role in industrial removal of dyes.
KW - Catalytic activity
KW - Electrospun materials
KW - Enzyme immobilisation
KW - Horseradish peroxidase
KW - Malachite Green
KW - Reactive Black 5
U2 - 10.1016/j.procbio.2020.11.015
DO - 10.1016/j.procbio.2020.11.015
M3 - Journal article
AN - SCOPUS:85097467959
SN - 1359-5113
VL - 102
SP - 10
EP - 21
JO - Process Biochemistry
JF - Process Biochemistry
ER -