HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

Dorota A Rzechonek, Mateusz Szczepańczyk, Guokun Wang, Irina Borodina, Aleksandra M. Mirończuk*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl-hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism.
Original languageEnglish
Article number1424
Issue number12
Number of pages15
Publication statusPublished - 2020


  • Yarrowia lipolytica
  • Erythritol
  • Hyperosmotic stress response
  • Osmolytes

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