Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

Ioannis Mougiakos, Elleke F. Bosma, Joyshree Ganguly , John van der Oost, Richard van Kranenburg*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

High engineering efficiencies are required for industrial strain development. Due to its user-friendliness and its stringency, CRISPR-Cas-based technologies have strongly increased genome engineering efficiencies in bacteria. This has enabled more rapid metabolic engineering of both the model host Escherichia coli and non-model organisms like Clostridia, Bacilli, Streptomycetes and cyanobacteria, opening new possibilities to use these organisms as improved cell factories. The discovery of novel Cas9-like systems from diverse microbial environments will extend the repertoire of applications and broaden the range of organisms in which it can be used to create novel production hosts. This review analyses the current status of prokaryotic metabolic engineering towards the production of biotechnologically relevant products, based on the exploitation of different CRISPR-related DNA/RNA endonuclease variants.
Original languageEnglish
JournalCurrent Opinion in Biotechnology
Volume50
Pages (from-to)146-157
ISSN0958-1669
DOIs
Publication statusPublished - 2018

Bibliographical note

c 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).

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