High-throughput sequencing-based investigation of viruses in human cancers by multi-enrichment approach

Research output: Contribution to journalJournal article – Annual report year: 2019Researchpeer-review

DOI

  • Author: Mollerup, Sarah

    University of Copenhagen, Denmark

  • Author: Asplund, Maria

    University of Copenhagen, Denmark

  • Author: Friis-Nielsen, Jens

    Department of Health Technology, Technical University of Denmark

  • Author: Kjartansdóttir, Kristín Rós

    University of Copenhagen, Denmark

  • Author: Fridholm, Helena

    University of Copenhagen, Denmark

  • Author: Hansen, Thomas Arn

    University of Copenhagen, Denmark

  • Author: Herrera, José Alejandro Romero

    Department of Health Technology, Technical University of Denmark

  • Author: Barnes, Christopher James

    University of Copenhagen, Denmark

  • Author: Jensen, Randi Holm

    University of Copenhagen, Denmark

  • Author: Richter, Stine Raith

    University of Copenhagen, Denmark

  • Author: Nielsen, Ida Broman

    University of Copenhagen, Denmark

  • Author: Pietroni, Carlotta

    University of Copenhagen, Denmark

  • Author: Alquezar-Planas, David E.

    University of Copenhagen, Denmark

  • Author: Rey-Iglesia, Alba

    University of Copenhagen, Denmark

  • Author: Olsen, Pernille V. S.

    University of Copenhagen, Denmark

  • Author: Rajpert-De Meyts, Ewa

    Copenhagen University Hospital, Denmark

  • Author: Groth-Pedersen, Line

    Copenhagen University Hospital, Denmark

  • Author: von Buchwald, Christian

    Copenhagen University Hospital, Denmark

  • Author: Jensen, David H.

    Copenhagen University Hospital, Denmark

  • Author: Gniadecki, Robert

    Bispebjerg University Hospital, Denmark

  • Author: Høgdall, Estrid

    University Hospital Herlev, Denmark

  • Author: Langhoff, Jill Levin

    University Hospital Herlev, Denmark

  • Author: Pete, Imre

    National Institute of Oncology, Hungary

  • Author: Vereczkey, Ildikó

    National Institute of Oncology, Hungary

  • Author: Baranyai, Zsolt

    Semmelweis University, Hungary

  • Author: Dybkær, Karen

    Aalborg University, Denmark

  • Author: Johnsen, Hans Erik

    Aarhus University Hospital, Denmark

  • Author: Steiniche, Torben

    Aarhus University Hospital, Denmark

  • Author: Hokland, Peter

    Aarhus University Hospital, Denmark

  • Author: Rosenberg, Jacob

    University Hospital Herlev, Denmark

  • Author: Baandrup, Ulrik

    Aalborg University Hospital, Denmark

  • Author: Sicheritz-Pontén, Thomas

    Department of Health Technology, Technical University of Denmark

  • Author: Willerslev, Eske

    University of Copenhagen, Denmark

  • Author: Brunak, Søren

    Integrative Systems Biology, Bioinformatics, Department of Health Technology, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Lund, Ole

    Technical University of Denmark, Denmark

  • Author: Mourier, Tobias

    University of Copenhagen, Denmark

  • Author: Vinner, Lasse

    University of Copenhagen, Denmark

  • Author: Izarzugaza, Jose M. G.

    Integrative Systems Biology, Bioinformatics, Department of Health Technology, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Nielsen, Lars Peter

    Statens Serum Institut, Denmark

  • Author: Hansen, Anders Johannes

    University of Copenhagen, Denmark

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Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data. Here we applied a diverse selection of pre-sequencing enrichment methods targeting all major viral groups, to characterise the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing we generated 710 datasets constituting 57 billion sequencing reads. Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveals a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained one or more viruses, however, no link between specific viruses and cancer types were found. Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
Original languageEnglish
JournalJournal of Infectious Diseases
ISSN0022-1899
DOIs
Publication statusAccepted/In press - 2019
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Cancer, Virus, Virome, High-throughput sequencing, Next generation sequencing, Human, Enrichment, In-depth analysis, Artefacts

ID: 184724469