High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli

E. Hiszczynska-Sawicka, A. Brillowska-Dabrowska, Slawomir Dabrowski, H. Pietkiewicz, P. Myjak, J. Kur

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This report describes a simple, highly efficient and reproducible method for obtaining large quantities of highly pure recombinant Toxoplasma gondii antigens, which can be used for diagnostic application. The obtained T gondii SAG1, GRA1, and GRA7 antigens (as fusion proteins), expressed in Escherichia coli, contained polyhistidine tags at the N- and C-ends that allowed single-step isolation by metal-affinity chromatography on Ni2+-IDA-Sepharose columns. The immunoreactivity of the recombinant antigens was tested in an enzyme-linked immunosorbent assay (ELISA) format for potential application in the serodiagnosis of T gondii infection. (C) 2002 Elsevier Science (USA). All rights reserved.
    Original languageEnglish
    JournalProtein Expression and Purification
    Volume27
    Issue number1
    Pages (from-to)150-157
    ISSN1046-5928
    DOIs
    Publication statusPublished - 2003

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