High-throughput, temperature-controlled microchannel acoustophoresis device made with rapid prototyping

Jonathan D Adams, Christian L. Ebbesen, Rune Barnkob, Allen H J Yang, H Tom Soh, Henrik Bruus

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We report a temperature-controlled microfluidic acoustophoresis device capable of separating particles and transferring blood cells from undiluted whole human blood at a volume throughput greater than 1 L h−1. The device is fabricated from glass substrates and polymer sheets in microscope-slide format using low-cost, rapid-prototyping techniques. This high-throughput acoustophoresis chip (HTAC) utilizes a temperature-stabilized, standing ultrasonic wave, which imposes differential acoustic radiation forces that can separate particles according to size, density and compressibility. The device proved capable of separating a mixture of 10- and 2-μm-diameter polystyrene beads with a sorting efficiency of 0.8 at a flow rate of 1 L h−1. As a first step toward biological applications, the HTAC was also tested in processing whole human blood and proved capable of transferring blood cells from undiluted whole human blood with an efficiency of 0.95 at 1 L h−1 and 0.82 at 2 L h−1.

    Original languageEnglish
    JournalJournal of Micromechanics and Microengineering
    Volume22
    Issue number7
    Pages (from-to)Paper 075017
    Number of pages8
    ISSN0960-1317
    DOIs
    Publication statusPublished - 2012

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