High-throughput screening of tick-borne pathogens in Europe

Lorraine Michelet, Sabine Delannoy, Elodie Devillers, Gerald Umhang, Anna Aspan, Mikael Juremalm, Jan Chirico, Fimme Jan van der Wal, Thomas Peter Boye Pihl, Kirstine Klitgaard Schou, Rene Bødker, Patrick Fach, Sara Moutailler

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    Abstract

    Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was conducted on 7050 Ixodes ricinus nymphs collected from France, Denmark, and the Netherlands using a powerful new high-throughput approach. This advanced methodology permitted the simultaneous detection of 25 bacterial, and 12 parasitic species (including; Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia, and Theileria genus) across 94 samples. We successfully determined the prevalence of expected (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Candidatus Neoehrlichia mikurensis, Babesia divergens, Babesia venatorum), unexpected (Borrelia miyamotoi) and rare (Bartonella henselae) pathogens in the three European countries. Moreover we detected Borrelia spielmanii, Borrelia miyamotoi, Babesia divergens, and Babesia venatorum for the first time in Danish ticks. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens, and which can also be customized to monitor emerging diseases.
    Original languageEnglish
    Article number103
    JournalFrontiers in Cellular and Infection Microbiology
    Volume4
    Issue number103
    Number of pages13
    ISSN2235-2988
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Tick borne diseases
    • Molecular epidemiology
    • Surveillance
    • Europe
    • Microfluidic analyses

    Cite this

    Michelet, L., Delannoy, S., Devillers, E., Umhang, G., Aspan, A., Juremalm, M., Chirico, J., van der Wal, F. J., Pihl, T. P. B., Schou, K. K., Bødker, R., Fach, P., & Moutailler, S. (2014). High-throughput screening of tick-borne pathogens in Europe. Frontiers in Cellular and Infection Microbiology, 4(103), [103]. https://doi.org/10.3389/fcimb.2014.00103