High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Cristina Isabel Amador, Rune Overlund Stannius, Henriette Lyng Røder*, Mette Burmølle*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

86 Downloads (Pure)

Abstract

The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
Original languageEnglish
Article number106343
JournalJournal of Microbiological methods
Volume190
Number of pages5
ISSN0167-7012
DOIs
Publication statusPublished - 2021

Keywords

  • Biofilm
  • High throughput assay
  • Mixed species
  • Reproductubility
  • Imaging
  • Cytation5
  • Crystal violet
  • Fluorescence labelling

Fingerprint

Dive into the research topics of 'High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging'. Together they form a unique fingerprint.

Cite this