High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Cristina Isabel Amador; Rune Overlund Stannius; Henriette Lyng Røder; Mette Burmølle

doi:10.1016/j.mimet.2021.106343

High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Cristina Isabel Amador, Rune Overlund Stannius, Henriette Lyng Røder^*, Mette Burmølle^*

^*Corresponding author for this work

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

Original language	English
Article number	106343
Journal	Journal of Microbiological Methods
Volume	190
Number of pages	5
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2021.106343
Publication status	Published - 2021

Keywords

Biofilm
High throughput assay
Mixed species
Reproductubility
Imaging
Cytation5
Crystal violet
Fluorescence labelling

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abstract = "The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.",

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AU - Amador, Cristina Isabel

AU - Stannius, Rune Overlund

AU - Røder, Henriette Lyng

AU - Burmølle, Mette

PY - 2021

Y1 - 2021

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AB - The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

KW - Biofilm

KW - High throughput assay

KW - Mixed species

KW - Reproductubility

KW - Imaging

KW - Cytation5

KW - Crystal violet

KW - Fluorescence labelling

U2 - 10.1016/j.mimet.2021.106343

DO - 10.1016/j.mimet.2021.106343

M3 - Journal article

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

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High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Abstract

Keywords

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