High throughput production of mouse monoclonal antibodies using antigen microarrays

Federico De Masi, P. Chiarella, H. Wilhelm, M. Massimi, B. Bullard, W. Ansorge, A. Sawyer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Recent advances in proteomics research underscore the increasing need for high-affinity monoclonal antibodies, which are still generated with lengthy, low-throughput antibody production techniques. Here we present a semi-automated, high-throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6-10 wk using multiplexed immunisations, automated fusion and cell-culture, and a novel antigen-coated microarray-screening assay. In a large-scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.
    Original languageEnglish
    JournalProteomics
    Volume5
    Issue number16
    Pages (from-to)4070-4081
    ISSN1615-9853
    DOIs
    Publication statusPublished - 2005

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