TY - JOUR
T1 - High throughput production of mouse monoclonal antibodies using antigen microarrays
AU - De Masi, Federico
AU - Chiarella, P.
AU - Wilhelm, H.
AU - Massimi, M.
AU - Bullard, B.
AU - Ansorge, W.
AU - Sawyer, A.
PY - 2005
Y1 - 2005
N2 - Recent advances in proteomics research underscore the increasing need for high-affinity monoclonal antibodies, which are still generated with lengthy, low-throughput antibody production techniques. Here we present a semi-automated, high-throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6-10 wk using multiplexed immunisations, automated fusion and cell-culture, and a novel antigen-coated microarray-screening assay. In a large-scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.
AB - Recent advances in proteomics research underscore the increasing need for high-affinity monoclonal antibodies, which are still generated with lengthy, low-throughput antibody production techniques. Here we present a semi-automated, high-throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6-10 wk using multiplexed immunisations, automated fusion and cell-culture, and a novel antigen-coated microarray-screening assay. In a large-scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.
U2 - 10.1002/pmic.200401279
DO - 10.1002/pmic.200401279
M3 - Journal article
C2 - 16254927
SN - 1615-9853
VL - 5
SP - 4070
EP - 4081
JO - Proteomics
JF - Proteomics
IS - 16
ER -