High-throughput microarray analysis of pectic polymers by enzymatic epitope deletion

Jens Øbro, Iben Sørensen, Isabel Møller, Michael Skjøt, Jørn Dalgaard Mikkelsen, William G.T. Willats

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Pectic polysaccharides are abundant plant cell wall glycan polymers and are also widely used as functional food ingredients. The fine structure of pectins, and especially the degree and pattern of methyl-esterification of the homogalacturonan (HG) backbone, play a major part in determining functional properties. We have developed a high-throughput method of analysing pectic polymers by combining micro array-technology, pectin lyase (PL) and anti-HG monoclonal antibodies (mAbs). The technique demonstrates that pectins immobilised in a microarray format are effective substrates for PL, and that epitope loss or degradation can be tracked by the deletion of mAb binding. (c) 2007 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Polymers
Volume70
Issue number1
Pages (from-to)77-81
ISSN0144-8617
DOIs
Publication statusPublished - 2007
Externally publishedYes

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