TY - JOUR
T1 - High-throughput microarray analysis of pectic polymers by enzymatic epitope deletion
AU - Øbro, Jens
AU - Sørensen, Iben
AU - Møller, Isabel
AU - Skjøt, Michael
AU - Mikkelsen, Jørn Dalgaard
AU - Willats, William G.T.
PY - 2007
Y1 - 2007
N2 - Pectic polysaccharides are abundant plant cell wall glycan polymers and are also widely used as functional food ingredients. The fine structure of pectins, and especially the degree and pattern of methyl-esterification of the homogalacturonan (HG) backbone, play a major part in determining functional properties. We have developed a high-throughput method of analysing pectic polymers by combining micro array-technology, pectin lyase (PL) and anti-HG monoclonal antibodies (mAbs). The technique demonstrates that pectins immobilised in a microarray format are effective substrates for PL, and that epitope loss or degradation can be tracked by the deletion of mAb binding. (c) 2007 Elsevier Ltd. All rights reserved.
AB - Pectic polysaccharides are abundant plant cell wall glycan polymers and are also widely used as functional food ingredients. The fine structure of pectins, and especially the degree and pattern of methyl-esterification of the homogalacturonan (HG) backbone, play a major part in determining functional properties. We have developed a high-throughput method of analysing pectic polymers by combining micro array-technology, pectin lyase (PL) and anti-HG monoclonal antibodies (mAbs). The technique demonstrates that pectins immobilised in a microarray format are effective substrates for PL, and that epitope loss or degradation can be tracked by the deletion of mAb binding. (c) 2007 Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.carbpol.2007.03.008
DO - 10.1016/j.carbpol.2007.03.008
M3 - Journal article
SN - 0144-8617
VL - 70
SP - 77
EP - 81
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 1
ER -