High throughput label-free platform for statistical bio-molecular sensing

Filippo Bosco, En-Te Hwu, Ching-Hsiu Chen, Stephan Sylvest Keller, Michael Bache, Mogens Havsteen Jakobsen, Ing-Shouh Hwang, Anja Boisen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.
    Original languageEnglish
    JournalLab on a Chip
    Volume11
    Issue number14
    Pages (from-to)2411-2416
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2011

    Cite this

    Bosco, Filippo ; Hwu, En-Te ; Chen, Ching-Hsiu ; Keller, Stephan Sylvest ; Bache, Michael ; Jakobsen, Mogens Havsteen ; Hwang, Ing-Shouh ; Boisen, Anja. / High throughput label-free platform for statistical bio-molecular sensing. In: Lab on a Chip. 2011 ; Vol. 11, No. 14. pp. 2411-2416.
    @article{a5514ad4f94a444aa3cd0481a2827fc0,
    title = "High throughput label-free platform for statistical bio-molecular sensing",
    abstract = "Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.",
    author = "Filippo Bosco and En-Te Hwu and Ching-Hsiu Chen and Keller, {Stephan Sylvest} and Michael Bache and Jakobsen, {Mogens Havsteen} and Ing-Shouh Hwang and Anja Boisen",
    year = "2011",
    doi = "10.1039/c1lc20116f",
    language = "English",
    volume = "11",
    pages = "2411--2416",
    journal = "Lab on a Chip",
    issn = "1473-0197",
    publisher = "Royal Society of Chemistry",
    number = "14",

    }

    High throughput label-free platform for statistical bio-molecular sensing. / Bosco, Filippo; Hwu, En-Te; Chen, Ching-Hsiu; Keller, Stephan Sylvest; Bache, Michael; Jakobsen, Mogens Havsteen; Hwang, Ing-Shouh; Boisen, Anja.

    In: Lab on a Chip, Vol. 11, No. 14, 2011, p. 2411-2416.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - High throughput label-free platform for statistical bio-molecular sensing

    AU - Bosco, Filippo

    AU - Hwu, En-Te

    AU - Chen, Ching-Hsiu

    AU - Keller, Stephan Sylvest

    AU - Bache, Michael

    AU - Jakobsen, Mogens Havsteen

    AU - Hwang, Ing-Shouh

    AU - Boisen, Anja

    PY - 2011

    Y1 - 2011

    N2 - Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

    AB - Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

    U2 - 10.1039/c1lc20116f

    DO - 10.1039/c1lc20116f

    M3 - Journal article

    VL - 11

    SP - 2411

    EP - 2416

    JO - Lab on a Chip

    JF - Lab on a Chip

    SN - 1473-0197

    IS - 14

    ER -