Mycotoxins are toxic secondary metabolites produced by fungi, found in a variety of food sources and cause diseases and death in both humans and animals. On-site and easy to use detection would be significant improvement considering the currently used methods. We present an approach, based on surface-enhanced Raman spectroscopy (SERS), where label-free detection of Ochratoxin A (OTA) was achieved in a multiwell SERS detection platform. When quantifying OTA in wine samples the SERS-based detection was used in combination with high throughput supported liquid membrane (SLM) extraction. The obtained detection limit in white wine was 115 ppb and we found that the SERS-based quantification is comparable with LC-MS. However, we also found that the matrix effect from red wine was interfering with both SERS and LC-MS detection. The SLM-SERS method provides a time and cost effective approach for OTA detection, which is a clear advantage over other extraction and direct detection approaches.
- Ag SERS substrate
- Direct label-free detection
- Ochratoxin A
- Parallel artificial liquid membrane extraction
Rostami, S., Zor, K., Zhai, D. S., Viehrig, M., Morelli, L., Mehdinia, A., Smedsgaard, J., Rindzevicius, T., & Boisen, A. (2020). High-throughput label-free detection of Ochratoxin A in wine using supported liquid membrane extraction and Ag-capped silicon nanopillar SERS substrates. Food Control, 113, . https://doi.org/10.1016/j.foodcont.2020.107183