The invention relates to a recombinant microbial cell comprising a selection platform for screening for a protease inhibitor, wherein the platform comprises transgenes encoding a protease having selective peptide bond cleavage activity at a recognition site amino acid sequence; and transgenes encoding polypeptides conferring resistance to microbial growth inhibitors; wherein the polypeptides comprise the recognition site amino acid sequence cleavable by the protease. Protease inhibitors are detected by their ability to inhibit protease specific cleavage and inactivation of the polypeptides whose activity is required for conferring resistance to the microbial growth inhibitors. The invention further relates to recombinant microbial host cell libraries of metagenomic DNA that further comprise the selection platform; and the use of a recombinant microbial cell comprising the selection platform for screening for a protease inhibitor.
|IPC||C12Q 1/ 37 A I|
|Country/Territory||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 2 Nov 2017|