High sensitivity point-of-care device for direct virus diagnostics

Katrine Kiilerich-Pedersen, Johannes Dapra, Solène Cherré, Noemi Rozlosnik

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15. min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles. © 2013 Elsevier B.V.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume49
    Pages (from-to)374-379
    ISSN0956-5663
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Electrochemical impedance spectroscopy
    • Fluidic devices
    • Microelectrodes
    • Polymers
    • Viruses
    • Conductive polymer
    • PEDOT-OH:TsO
    • Electrochemical impedance Ćspectroscopy
    • (EIS)
    • DNA aptamer
    • Influenza A virus (H1N1)
    • AFM
    • Point-of-care (POC)

    Cite this

    Kiilerich-Pedersen, Katrine ; Dapra, Johannes ; Cherré, Solène ; Rozlosnik, Noemi. / High sensitivity point-of-care device for direct virus diagnostics. In: Biosensors and Bioelectronics. 2013 ; Vol. 49. pp. 374-379.
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    abstract = "Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15. min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles. {\circledC} 2013 Elsevier B.V.",
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    Kiilerich-Pedersen, K, Dapra, J, Cherré, S & Rozlosnik, N 2013, 'High sensitivity point-of-care device for direct virus diagnostics', Biosensors and Bioelectronics, vol. 49, pp. 374-379. https://doi.org/10.1016/j.bios.2013.05.046

    High sensitivity point-of-care device for direct virus diagnostics. / Kiilerich-Pedersen, Katrine; Dapra, Johannes; Cherré, Solène; Rozlosnik, Noemi.

    In: Biosensors and Bioelectronics, Vol. 49, 2013, p. 374-379.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    T1 - High sensitivity point-of-care device for direct virus diagnostics

    AU - Kiilerich-Pedersen, Katrine

    AU - Dapra, Johannes

    AU - Cherré, Solène

    AU - Rozlosnik, Noemi

    PY - 2013

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    AB - Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15. min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles. © 2013 Elsevier B.V.

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    KW - Microelectrodes

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    KW - Conductive polymer

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    KW - Electrochemical impedance Ćspectroscopy

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    KW - DNA aptamer

    KW - Influenza A virus (H1N1)

    KW - AFM

    KW - Point-of-care (POC)

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    JO - Biosensors and Bioelectronics

    JF - Biosensors and Bioelectronics

    SN - 0956-5663

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