High-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus and characterization of its interaction with the bifunctional HPr kinase/phosphorylase

T. Maurer, Sebastian Meier, N. Kachel, CE Munte, S. Hasenbein, B. Koch, W. Hengstenberg, HR Kalbitzer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A high-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus was obtained by heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopy on the basis of 1,766 structural restraints. Twenty-three hydrogen bonds in HPr could be directly detected by polarization transfer from the amide nitrogen to the carbonyl carbon involved in the hydrogen bond. Differential line broadening was used to characterize the interaction of HPr with the HPr kinase/phosphorylase (HPrK/P) of Staphylococcus xylosus, which is responsible for phosphorylation-dephosphorylation of the hydroxyl group of the regulatory serine residue at position 46. The dissociation constant K-d was determined to be 0.10 +/- 0.02 mM at 303 K from the NMR data, assuming independent binding. The data are consistent with a stoichiometry of 1 HPr molecule per HPrK/P monomer in solution. Using transversal relaxation optimized spectroscopy-heteronuclear single quantum correlation, we mapped the interaction site of the two proteins in the 330-kDa complex. As expected, it covers the region around Ser46 and the small helix b following this residue. In addition, HPrK/P also binds to the second phosphorylation site of HPr at position 15. This interaction may be essential for the recognition of the phosphorylation state of His15 and the phosphorylation-dependent regulation of the kinase/phosphorylase activity. In accordance with this observation, the recently published X-ray structure of the HPr/HPrK core protein complex from Lactobacillus casei shows interactions with the two phosphorylation sites. However, the NMR data also suggest differences for the full-length protein from S. xylosus: there are no indications for an interaction with the residues preceding the regulatory Ser46 residue (Thr41 to Lys45) in the protein of S.xylosus. In contrast, it seems to interact with the C-terminal helix of HPr in solution, an interaction which is not observed for the complex of HPr with the core of HPrK/P of L. casei in crystals.
Original languageEnglish
JournalJournal of Bacteriology
Volume186
Issue number17
Pages (from-to)5906-5918
Number of pages13
ISSN0021-9193
DOIs
Publication statusPublished - 2004
Externally publishedYes

Keywords

  • PHOSPHORYLATION
  • Amino Acids
  • Bacterial Proteins
  • Lactobacillus casei
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • Phosphorylation
  • Protein Binding
  • Protein Interaction Mapping
  • Protein-Serine-Threonine Kinases
  • Staphylococcus aureus
  • EC 2.7.1.- Phosphoenolpyruvate Sugar Phosphotransferase System
  • EC 2.7.1.- phosphocarrier protein HPr
  • EC 2.7.11.1 HPr kinase
  • EC 2.7.11.1 Protein-Serine-Threonine Kinases
  • amide
  • carbon
  • carbonyl derivative
  • carrier protein
  • histidine containing phosphocarrier protein
  • hpr kinase phosphorylase
  • hydroxyl group
  • nitrogen
  • phosphorylase
  • serine
  • unclassified drug
  • article
  • dephosphorylation
  • dissociation constant
  • hydrogen bond
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • phosphorylation
  • polarization
  • priority journal
  • protein protein interaction
  • protein structure
  • Staphylococcus xylosus
  • stoichiometry
  • X ray
  • Bacteria (microorganisms)
  • Lactobacillus
  • Posibacteria
  • Staphylococcus
  • MICROBIOLOGY
  • CARBON CATABOLITE REPRESSION
  • SUGAR PHOSPHOTRANSFERASE SYSTEM
  • 2-DIMENSIONAL NMR-SPECTROSCOPY
  • SEQUENTIAL BACKBONE ASSIGNMENT
  • ISOTOPICALLY ENRICHED PROTEINS
  • X-RAY STRUCTURE
  • ESCHERICHIA-COLI
  • CHEMICAL-SHIFT
  • ENTEROCOCCUS-FAECALIS
  • SECONDARY STRUCTURE
  • bacterial physiology/biochemistry
  • comparative biochemistry
  • enzyme activity regulation
  • molecular interactions significance
  • protein phosphorylation
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Lactobacillus casei species
  • Gram-Positive Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Micrococcaceae [07702] Staphylococcus aureus species
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria common
  • amino acids
  • bifunctional enzymes functions, properties
  • bifunctional HPr kinase/phosphorylase activities, analysis, bacterial, functions, properties
  • enzymes functions, properties
  • histidine 71-00-1Q, 4998-57-6Q
  • histidine-containing phosphocarrier protein analysis, bacterial, functions, high resolution structure
  • proteins functions, properties
  • 10060, Biochemistry studies - General
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 31000, Physiology and biochemistry of bacteria
  • NMR spectroscopy laboratory techniques, spectrum analysis techniques
  • Biochemistry and Molecular Biophysics

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