High-resolution genomic fingerprinting of Campylobacter jejuni and Campylobacter coli by analysis of amplified fragment length polymorphisms

Branko Kokotovic, Stephen L.W. On

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A method for high-resolution genomic fingerprinting of the enteric pathogens Campylobacter jejuni and Campylobacter coli, based on the determination of amplified fragment length polymorphism, is described. The potential of this method for molecular epidemiological studies of these species is evaluated with 50 type, reference, and well-characterised field strains. Amplified fragment length polymorphism fingerprints comprised over 60 bands detected in the size range 35-500 bp. Groups of outbreak strains, replicate subcultures, and 'genetically identical' strains from humans, poultry and cattle, proved indistinguishable by amplified fragment length polymorphism fingerprinting, but were differentiated fi-om unrelated isolates. Previously unknown relationships between three hippurate-negative C. jejuni strains, and two C. coil var, hyoilei strains, were identified. These relationships corresponded to available epidemiological data. We conclude that this amplified fragment length polymorphism fingerprinting method may be a highly effective tool for molecular epidemiological studies of Campylobacter spp.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume173
    Issue number1
    Pages (from-to)77-84
    ISSN0378-1097
    DOIs
    Publication statusPublished - 1999

    Keywords

    • genomic fingerprinting
    • Campylobacter
    • molecular epidemiology
    • amplified fragment length polymorphism

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