High-performance liquid chromatography analysis methods developed for quantifying enzymatic esterification of flavonoids in ionic liquids

Bena-Marie Lue, Zheng Guo, X.B. Xu

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Methods using reversed-phase high-performance liquid chromatography (RP-HPLC) with ELSD were investigated to quantify enzymatic reactions of flavonoids with fatty acids in the presence of diverse room temperature ionic liquids (RTILs). A buffered salt (preferably triethylamine-acetate) was found essential for separation of flavonoids from strongly polar RTILs, whereby RTILs were generally visible as two major peaks identified based on an ion-pairing/exchanging hypothesis. C8 and C12 stationary phases were optimal while mobile phase pH (3-7) had only a minor influence on separation. The method developed was successfully applied for primary screening of RTILs (> 20), with in depth evaluation of substrates in 10 RTILs, for their evaluation as reaction media.
Original languageEnglish
JournalJournal of Chromatography A
Volume1198
Issue number1-2
Pages (from-to)107-114
ISSN0021-9673
DOIs
Publication statusPublished - 2008

Keywords

  • reversed-phase high-performance liquid chromatography
  • evaporative light scattering detection (ELSD)
  • enzymatic esterification
  • (RP-HPLC)
  • room temperature ionic liquids (RTIL)
  • flavonoids

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