High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples

M. J. Gosalbes, Y. Vallès, N. Jiménez-Hernández, C. Balle, P. Riva, S. Miravet-Verde, L. E. de Vries, S. Llop, Yvonne Agersø, S. J. Sørensen, F. Ballester, M. P. Francino

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The gastrointestinal tract (GIT) microbiota has been identified as an important reservoir of antibiotic resistance genes (ARGs) that can be horizontally transferred to pathogenic species. Maternal GIT microbes can be transmitted to the offspring, and recent work indicates that such transfer starts before birth. We have used culture-independent genetic screenings to explore whether ARGs are already present in the meconium accumulated in the GIT during fetal life and in feces of 1-week-old infants. We have analyzed resistance to β-lactam antibiotics (BLr) and tetracycline (Tcr), screening for a variety of genes conferring each. To evaluate whether ARGs could have been inherited by maternal transmission, we have screened perinatal fecal samples of the 1-week-old babies’ mothers, as well as a mother–infant series including meconium, fecal samples collected through the infant’s 1st year, maternal fecal samples and colostrum. Our results reveal a high prevalence of BLr and Tcr in both meconium and early fecal samples, implying that the GIT resistance reservoir starts to accumulate even before birth. We show that ARGs present in the mother may reach the meconium and colostrum and establish in the infant GIT, but also that some ARGs were likely acquired from other sources. Alarmingly, we identified in both meconium and 1-week-olds’ samples a particularly elevated prevalence of mecA (>45%), six-fold higher than that detected in the mothers. The mecA gene confers BLr to methicillin-resistant Staphylococcus aureus, and although its detection does not imply the presence of this pathogen, it does implicate the young infant’s GIT as a noteworthy reservoir of this gene.
Original languageEnglish
JournalJournal of Developmental Origins of Health and Disease
Volume7
Issue number1
Pages (from-to)35-44
Number of pages10
ISSN2040-1744
DOIs
Publication statusPublished - 2016

Keywords

  • Medicine (miscellaneous)
  • antibiotic resistance
  • gastrointestinal microbiota
  • mecA
  • meconium
  • tetracycline

Cite this

Gosalbes, M. J., Vallès, Y., Jiménez-Hernández, N., Balle, C., Riva, P., Miravet-Verde, S., ... Francino, M. P. (2016). High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples. Journal of Developmental Origins of Health and Disease, 7(1), 35-44. https://doi.org/10.1017/s2040174415001506
Gosalbes, M. J. ; Vallès, Y. ; Jiménez-Hernández, N. ; Balle, C. ; Riva, P. ; Miravet-Verde, S. ; de Vries, L. E. ; Llop, S. ; Agersø, Yvonne ; Sørensen, S. J. ; Ballester, F. ; Francino, M. P. / High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples. In: Journal of Developmental Origins of Health and Disease. 2016 ; Vol. 7, No. 1. pp. 35-44.
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abstract = "The gastrointestinal tract (GIT) microbiota has been identified as an important reservoir of antibiotic resistance genes (ARGs) that can be horizontally transferred to pathogenic species. Maternal GIT microbes can be transmitted to the offspring, and recent work indicates that such transfer starts before birth. We have used culture-independent genetic screenings to explore whether ARGs are already present in the meconium accumulated in the GIT during fetal life and in feces of 1-week-old infants. We have analyzed resistance to β-lactam antibiotics (BLr) and tetracycline (Tcr), screening for a variety of genes conferring each. To evaluate whether ARGs could have been inherited by maternal transmission, we have screened perinatal fecal samples of the 1-week-old babies’ mothers, as well as a mother–infant series including meconium, fecal samples collected through the infant’s 1st year, maternal fecal samples and colostrum. Our results reveal a high prevalence of BLr and Tcr in both meconium and early fecal samples, implying that the GIT resistance reservoir starts to accumulate even before birth. We show that ARGs present in the mother may reach the meconium and colostrum and establish in the infant GIT, but also that some ARGs were likely acquired from other sources. Alarmingly, we identified in both meconium and 1-week-olds’ samples a particularly elevated prevalence of mecA (>45{\%}), six-fold higher than that detected in the mothers. The mecA gene confers BLr to methicillin-resistant Staphylococcus aureus, and although its detection does not imply the presence of this pathogen, it does implicate the young infant’s GIT as a noteworthy reservoir of this gene.",
keywords = "Medicine (miscellaneous), antibiotic resistance, gastrointestinal microbiota, mecA, meconium, tetracycline",
author = "Gosalbes, {M. J.} and Y. Vall{\`e}s and N. Jim{\'e}nez-Hern{\'a}ndez and C. Balle and P. Riva and S. Miravet-Verde and {de Vries}, {L. E.} and S. Llop and Yvonne Agers{\o} and S{\o}rensen, {S. J.} and F. Ballester and Francino, {M. P.}",
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Gosalbes, MJ, Vallès, Y, Jiménez-Hernández, N, Balle, C, Riva, P, Miravet-Verde, S, de Vries, LE, Llop, S, Agersø, Y, Sørensen, SJ, Ballester, F & Francino, MP 2016, 'High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples', Journal of Developmental Origins of Health and Disease, vol. 7, no. 1, pp. 35-44. https://doi.org/10.1017/s2040174415001506

High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples. / Gosalbes, M. J.; Vallès, Y.; Jiménez-Hernández, N.; Balle, C.; Riva, P.; Miravet-Verde, S.; de Vries, L. E.; Llop, S.; Agersø, Yvonne; Sørensen, S. J.; Ballester, F.; Francino, M. P.

In: Journal of Developmental Origins of Health and Disease, Vol. 7, No. 1, 2016, p. 35-44.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - High frequencies of antibiotic resistance genes in infants’ meconium and early fecal samples

AU - Gosalbes, M. J.

AU - Vallès, Y.

AU - Jiménez-Hernández, N.

AU - Balle, C.

AU - Riva, P.

AU - Miravet-Verde, S.

AU - de Vries, L. E.

AU - Llop, S.

AU - Agersø, Yvonne

AU - Sørensen, S. J.

AU - Ballester, F.

AU - Francino, M. P.

PY - 2016

Y1 - 2016

N2 - The gastrointestinal tract (GIT) microbiota has been identified as an important reservoir of antibiotic resistance genes (ARGs) that can be horizontally transferred to pathogenic species. Maternal GIT microbes can be transmitted to the offspring, and recent work indicates that such transfer starts before birth. We have used culture-independent genetic screenings to explore whether ARGs are already present in the meconium accumulated in the GIT during fetal life and in feces of 1-week-old infants. We have analyzed resistance to β-lactam antibiotics (BLr) and tetracycline (Tcr), screening for a variety of genes conferring each. To evaluate whether ARGs could have been inherited by maternal transmission, we have screened perinatal fecal samples of the 1-week-old babies’ mothers, as well as a mother–infant series including meconium, fecal samples collected through the infant’s 1st year, maternal fecal samples and colostrum. Our results reveal a high prevalence of BLr and Tcr in both meconium and early fecal samples, implying that the GIT resistance reservoir starts to accumulate even before birth. We show that ARGs present in the mother may reach the meconium and colostrum and establish in the infant GIT, but also that some ARGs were likely acquired from other sources. Alarmingly, we identified in both meconium and 1-week-olds’ samples a particularly elevated prevalence of mecA (>45%), six-fold higher than that detected in the mothers. The mecA gene confers BLr to methicillin-resistant Staphylococcus aureus, and although its detection does not imply the presence of this pathogen, it does implicate the young infant’s GIT as a noteworthy reservoir of this gene.

AB - The gastrointestinal tract (GIT) microbiota has been identified as an important reservoir of antibiotic resistance genes (ARGs) that can be horizontally transferred to pathogenic species. Maternal GIT microbes can be transmitted to the offspring, and recent work indicates that such transfer starts before birth. We have used culture-independent genetic screenings to explore whether ARGs are already present in the meconium accumulated in the GIT during fetal life and in feces of 1-week-old infants. We have analyzed resistance to β-lactam antibiotics (BLr) and tetracycline (Tcr), screening for a variety of genes conferring each. To evaluate whether ARGs could have been inherited by maternal transmission, we have screened perinatal fecal samples of the 1-week-old babies’ mothers, as well as a mother–infant series including meconium, fecal samples collected through the infant’s 1st year, maternal fecal samples and colostrum. Our results reveal a high prevalence of BLr and Tcr in both meconium and early fecal samples, implying that the GIT resistance reservoir starts to accumulate even before birth. We show that ARGs present in the mother may reach the meconium and colostrum and establish in the infant GIT, but also that some ARGs were likely acquired from other sources. Alarmingly, we identified in both meconium and 1-week-olds’ samples a particularly elevated prevalence of mecA (>45%), six-fold higher than that detected in the mothers. The mecA gene confers BLr to methicillin-resistant Staphylococcus aureus, and although its detection does not imply the presence of this pathogen, it does implicate the young infant’s GIT as a noteworthy reservoir of this gene.

KW - Medicine (miscellaneous)

KW - antibiotic resistance

KW - gastrointestinal microbiota

KW - mecA

KW - meconium

KW - tetracycline

U2 - 10.1017/s2040174415001506

DO - 10.1017/s2040174415001506

M3 - Journal article

VL - 7

SP - 35

EP - 44

JO - Journal of Developmental Origins of Health and Disease

JF - Journal of Developmental Origins of Health and Disease

SN - 2040-1744

IS - 1

ER -