High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge

Yingyu Law, Artur Matysik, Xueming Chen, Sara Swa Thi, Thi Quynh Ngoc Nguyen, Guanglei Qiu, Gayathri Natarajan, Rohan B H Williams, Bing-Jie Ni, Thomas William Seviour, Stefan Wuertz*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.
Original languageEnglish
JournalEnvironmental Science and Technology
Volume53
Issue number14
Pages (from-to)8157-8166
ISSN0013-936X
DOIs
Publication statusPublished - 2019

Cite this

Law, Yingyu ; Matysik, Artur ; Chen, Xueming ; Swa Thi, Sara ; Ngoc Nguyen, Thi Quynh ; Qiu, Guanglei ; Natarajan, Gayathri ; Williams, Rohan B H ; Ni, Bing-Jie ; Seviour, Thomas William ; Wuertz, Stefan. / High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge. In: Environmental Science and Technology. 2019 ; Vol. 53, No. 14. pp. 8157-8166.
@article{e1ef65b3d9b643e2ac6aa5031812aa70,
title = "High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge",
abstract = "A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.",
author = "Yingyu Law and Artur Matysik and Xueming Chen and {Swa Thi}, Sara and {Ngoc Nguyen}, {Thi Quynh} and Guanglei Qiu and Gayathri Natarajan and Williams, {Rohan B H} and Bing-Jie Ni and Seviour, {Thomas William} and Stefan Wuertz",
year = "2019",
doi = "10.1021/acs.est.9b00955",
language = "English",
volume = "53",
pages = "8157--8166",
journal = "Environmental Science & Technology (Washington)",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "14",

}

Law, Y, Matysik, A, Chen, X, Swa Thi, S, Ngoc Nguyen, TQ, Qiu, G, Natarajan, G, Williams, RBH, Ni, B-J, Seviour, TW & Wuertz, S 2019, 'High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge', Environmental Science and Technology, vol. 53, no. 14, pp. 8157-8166. https://doi.org/10.1021/acs.est.9b00955

High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge. / Law, Yingyu; Matysik, Artur; Chen, Xueming; Swa Thi, Sara; Ngoc Nguyen, Thi Quynh; Qiu, Guanglei; Natarajan, Gayathri; Williams, Rohan B H; Ni, Bing-Jie; Seviour, Thomas William; Wuertz, Stefan.

In: Environmental Science and Technology, Vol. 53, No. 14, 2019, p. 8157-8166.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - High Dissolved Oxygen Selection against Nitrospira Sublineage I in Full-Scale Activated Sludge

AU - Law, Yingyu

AU - Matysik, Artur

AU - Chen, Xueming

AU - Swa Thi, Sara

AU - Ngoc Nguyen, Thi Quynh

AU - Qiu, Guanglei

AU - Natarajan, Gayathri

AU - Williams, Rohan B H

AU - Ni, Bing-Jie

AU - Seviour, Thomas William

AU - Wuertz, Stefan

PY - 2019

Y1 - 2019

N2 - A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.

AB - A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.

U2 - 10.1021/acs.est.9b00955

DO - 10.1021/acs.est.9b00955

M3 - Journal article

VL - 53

SP - 8157

EP - 8166

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

SN - 0013-936X

IS - 14

ER -