Heterotrophs are key contributors to nitrous oxide production in mixed liquor under low C-to-N ratios during nitrification - batch experiments and modelling

Carlos Domingo Felez, Carles Pellicer i Nàcher, Morten S. Petersen, Marlene Mark Jensen, Benedek G. Plósz, Barth F. Smets

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Abstract

Nitrous oxide (N2O), a by-product of biological nitrogen removal during wastewater treatment, is produced by ammonia-oxidizing bacteria (AOB) and heterotrophic denitrifying bacteria (HB). Mathematical models are used to predict N2O emissions, often including AOB as the main N2O producer. Several model structures have been proposed without consensus calibration procedures. Here, we present a new experimental design that was used to calibrate AOB-driven N2O dynamics of a mixed culture. Even though AOB activity was favoured with respect to HB, oxygen uptake rates indicated HB activity. Hence, rigorous experimental design for calibration of autotrophic N2O production from mixed cultures is essential. The proposed N2O production pathways were examined using five alternative process models confronted with experimental data inferred. Individually, the autotrophic and heterotrophic denitrification pathway could describe the observed data. In the best-fit model, which combined two denitrification pathways, the heterotrophic was stronger than the autotrophic contribution to N2O production. Importantly, the individual contribution of autotrophic and heterotrophic to the total N2O pool could not be unambiguously elucidated solely based on bulk N2O measurements. Data on NO would increase the practical identifiability of N2O production pathways.
Original languageEnglish
JournalBiotechnology and Bioengineering
Volume114
Issue number1
Pages (from-to)132-140
ISSN0006-3592
DOIs
Publication statusPublished - 2017

Cite this

@article{11c8f0e9898d45938fdf4192a4d6b4cc,
title = "Heterotrophs are key contributors to nitrous oxide production in mixed liquor under low C-to-N ratios during nitrification - batch experiments and modelling",
abstract = "Nitrous oxide (N2O), a by-product of biological nitrogen removal during wastewater treatment, is produced by ammonia-oxidizing bacteria (AOB) and heterotrophic denitrifying bacteria (HB). Mathematical models are used to predict N2O emissions, often including AOB as the main N2O producer. Several model structures have been proposed without consensus calibration procedures. Here, we present a new experimental design that was used to calibrate AOB-driven N2O dynamics of a mixed culture. Even though AOB activity was favoured with respect to HB, oxygen uptake rates indicated HB activity. Hence, rigorous experimental design for calibration of autotrophic N2O production from mixed cultures is essential. The proposed N2O production pathways were examined using five alternative process models confronted with experimental data inferred. Individually, the autotrophic and heterotrophic denitrification pathway could describe the observed data. In the best-fit model, which combined two denitrification pathways, the heterotrophic was stronger than the autotrophic contribution to N2O production. Importantly, the individual contribution of autotrophic and heterotrophic to the total N2O pool could not be unambiguously elucidated solely based on bulk N2O measurements. Data on NO would increase the practical identifiability of N2O production pathways.",
author = "{Domingo Felez}, Carlos and {Pellicer i N{\`a}cher}, Carles and Petersen, {Morten S.} and Jensen, {Marlene Mark} and Pl{\'o}sz, {Benedek G.} and Smets, {Barth F.}",
year = "2017",
doi = "10.1002/bit.26062",
language = "English",
volume = "114",
pages = "132--140",
journal = "Biotechnology and Bioengineering (Print)",
issn = "0006-3592",
publisher = "JohnWiley & Sons, Inc.",
number = "1",

}

Heterotrophs are key contributors to nitrous oxide production in mixed liquor under low C-to-N ratios during nitrification - batch experiments and modelling. / Domingo Felez, Carlos; Pellicer i Nàcher, Carles; Petersen, Morten S.; Jensen, Marlene Mark; Plósz, Benedek G.; Smets, Barth F.

In: Biotechnology and Bioengineering, Vol. 114, No. 1, 2017, p. 132-140.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Heterotrophs are key contributors to nitrous oxide production in mixed liquor under low C-to-N ratios during nitrification - batch experiments and modelling

AU - Domingo Felez, Carlos

AU - Pellicer i Nàcher, Carles

AU - Petersen, Morten S.

AU - Jensen, Marlene Mark

AU - Plósz, Benedek G.

AU - Smets, Barth F.

PY - 2017

Y1 - 2017

N2 - Nitrous oxide (N2O), a by-product of biological nitrogen removal during wastewater treatment, is produced by ammonia-oxidizing bacteria (AOB) and heterotrophic denitrifying bacteria (HB). Mathematical models are used to predict N2O emissions, often including AOB as the main N2O producer. Several model structures have been proposed without consensus calibration procedures. Here, we present a new experimental design that was used to calibrate AOB-driven N2O dynamics of a mixed culture. Even though AOB activity was favoured with respect to HB, oxygen uptake rates indicated HB activity. Hence, rigorous experimental design for calibration of autotrophic N2O production from mixed cultures is essential. The proposed N2O production pathways were examined using five alternative process models confronted with experimental data inferred. Individually, the autotrophic and heterotrophic denitrification pathway could describe the observed data. In the best-fit model, which combined two denitrification pathways, the heterotrophic was stronger than the autotrophic contribution to N2O production. Importantly, the individual contribution of autotrophic and heterotrophic to the total N2O pool could not be unambiguously elucidated solely based on bulk N2O measurements. Data on NO would increase the practical identifiability of N2O production pathways.

AB - Nitrous oxide (N2O), a by-product of biological nitrogen removal during wastewater treatment, is produced by ammonia-oxidizing bacteria (AOB) and heterotrophic denitrifying bacteria (HB). Mathematical models are used to predict N2O emissions, often including AOB as the main N2O producer. Several model structures have been proposed without consensus calibration procedures. Here, we present a new experimental design that was used to calibrate AOB-driven N2O dynamics of a mixed culture. Even though AOB activity was favoured with respect to HB, oxygen uptake rates indicated HB activity. Hence, rigorous experimental design for calibration of autotrophic N2O production from mixed cultures is essential. The proposed N2O production pathways were examined using five alternative process models confronted with experimental data inferred. Individually, the autotrophic and heterotrophic denitrification pathway could describe the observed data. In the best-fit model, which combined two denitrification pathways, the heterotrophic was stronger than the autotrophic contribution to N2O production. Importantly, the individual contribution of autotrophic and heterotrophic to the total N2O pool could not be unambiguously elucidated solely based on bulk N2O measurements. Data on NO would increase the practical identifiability of N2O production pathways.

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