Heterologous production of polycyclopropanated fatty acids and their methyl esters in Streptomyces

Kevin Yin*, Pablo Cruz-Morales, Christopher M. Whitford, Jay D. Keasling*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Polycyclopropanated (POP) compounds show promise as fuels as their energy density can be greater than jet and rocket fuels in current use, but realizing their full potential requires significant development. This protocol guides the production of polycyclopropanated fatty acids in Streptomyces; POP production in another host remains to be demonstrated. This method can serve as a baseline for further development of POP as well as other polyketide products. For complete details on the use and execution of this protocol, please refer to Cruz-Morales et al. (2022).1

Original languageEnglish
Article number102190
JournalSTAR Protocols
Volume4
Issue number2
Number of pages23
ISSN2666-1667
DOIs
Publication statusPublished - 2023

Keywords

  • Biotechnology and Bioengineering
  • Chemistry
  • Energy
  • Mass Spectrometry
  • Microbiology
  • Molecular Biology

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